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2000 Fiscal Year Final Research Report Summary

Purification of lipid hydroperoxide reducing proteins from blood plasma, and analysis of its clinical roles

Research Project

Project/Area Number 10670146
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Pathological medical chemistry
Research InstitutionTokai University

Principal Investigator

YOSHIMURA Shinichi  Tokai University, School of Medicine, Assistant professor, 医学部, 講師 (30230808)

Project Period (FY) 1998 – 2000
KeywordsLipid hydroperoxide / Lipidperoxide reducing activity / Apolipoprotein B100 / Apolipoprotein A1 / Pre-eclampsia / Diabetes mellitus / Renal failure
Research Abstract

Plasma lipid hydroperoxide has been known as a risk factor for atherogenesis, because lipid peroxide can injury the endothelial cells. We found that human blood plasma contains lipid hydroperoxide reducing proteins other than plasma glutathione peroxidase, a major lipid hydroperoxide reducing enzyme. In this study, we initially purified this lipid hydroperoxide reducing protein from human blood plasma by using the combinations of ammonium sulfate fractionation, QAE sepharose, Heparin sepharose and gel filtrations column chromatography. Amino acids sequence analysis indicated that apolipoprotein A1 and B100 possess the lipid hydroperoxide reducing activity. The molecular mechanism of reduction of lipid peroxide by those apolipoproteins were investigated by using the synthetic oligopeptides, and revealed that methionine residues are oxidized to methionine-sulfoxide with concomitant reduction of lipid hydroperoxide to corresponding alcohols. To clarify the patho-physiological roles of the activity, we have developed an assay method to measure plasma lipid hydroperoxide reducing activity using synthetic 1-palmitoyl-2-linoleoil-Phosphatidylcholine hydroperoxide (PLPC-OOH) as the substrate. By using this method, PLPC-OOH reducing activities were determined in the 52 pregnant women, 11 pre-eclamptic, 18 male infertile, 24 chronic renal failure, and 34 diabetes mellitus patients. PLPC-OOH reducing activity was decreased in the pre-eclamptic patients to 90% than that of normal pregnancy, and increased in the patients with chronic renal failure and diabetes mellitus to about 1.5 fold than that of healthy controls. These data indicated that PLPC-OOH reducing activity may have important roles in the elimination of the plasma lipid hydroperoxide, and also suggested the activity may involved in the development of those diseases.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Mashima R: "Reduction of Phosphatidylcholine hydroperoxide by apolipoprotein A-1 : Purification of the hydroperoxide reducing proteins from human blood plasma."J.Lipid Res.. 39. 1133-1140 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Okada K: "Inflammatory cell-mediated tumor progression and minisatellite mutation correlated with the decrease of antioxidative enzymes in murine fibroblast."Br.J.Cancer. 79. 386-392 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mashima R: "Reduction of lipid hydroperoxide by apolipoprotein B-100"Biochem.Biophys.Res.Commun.. 259. 185-189 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 林田研一: "血漿過酸化リン脂質還元活性と妊娠中毒症"産婦人科の世界. 51. 939-943 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kurotani R: "Expression of Ptx1 in the adult rat pituitary glands and pituitary cell lines : hormone-secreting cells and folliculo-stellate-cells."Cell Tissue Res.. 298. 55-61 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tamaki T: "Limited myogenic response to a single bout of weight-lifting exercise in old rats."Am.J.Physiol.(cell physiol). 278. C1143-C1152 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mashima R, Yamamoto Y, and Yoshimura S.: "Reduction of phosphatidylcholine hydroperoxide by apolipoprotein A-1 : Purification of the hydroperoxide reducing proteins from human blood plasma."J.Lipid Res.. 39. 1133-1140 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Okada K, Nakai T, Kobayashi T Shibata T, Tagami S, Kawakami Y, Kitazawa T, kominami R, Yoshimura S et al.: "Inflammatory cell-mediated tumor progression and minisatellite mutation correlate with the decrease of antioxidative enzymes in murine fibrosarcoma cells."Br.J.Cancer. 79. 386-392 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mashima R, Yoshimura S, and Yamamoto Y.: "Reduction of lipid hydroperoxide by apolipoprotein B-100."Biochem.Biophys.Res.Commun.. 259. 185-189 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hayashida K, Iwasaki K, Makino T, Yoshimura S, Yamamoto Y.: "Plasma lipid-peroxide reducing proteins and pre-eclampsia."World of Gynecology. 51 (10). 939-943 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kurotani R, Tahara S, Sanno N, Teramoto A, Mellon P.L, Inoue K, Yoshimura S, and Osamura R.Y.: "Expression of Ptx1 in the adult rat pituitary glands and pituitary cell lines : hormone-secreting cells and folliculo-stellate cells."Cell Tissue Res.. 298. 55-61 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tamaki T, Uchiyama S, Uchiyama Y, Akatasuka A, Yoshimura S, Roy R.R, Edgerton V.R.: "Limited myogenic response to a single bout of weight-lifting exercise in old rats."Am.J.Physiol.(Cell Physiol.). 278. C1143-C1152 (2000)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2002-03-26  

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