1999 Fiscal Year Final Research Report Summary
STUDIES ON ORIGIN AND PROPERTIES OF A HODGIN CELL LINE, HPL-Hod-2, WHICH PRODUCE CYTOPLASMIC IgG
| Project/Area Number |
10670159
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Human pathology
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| Research Institution | SHIMANE MEDICAL UNIVERSITY |
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Principal Investigator |
MORIKAWA Shigeru SHIMANE MEDICAL UNIVERSITY, PATHOLOGY 1ィイD1STィエD1 UNIT, PROFESSOR, 医学部, 教授 (80027094)
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| Project Period (FY) |
1998 – 1999
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| Keywords | HODGKIN'S CELL / CELL LINE / CELL ORIGIN / IMMUNOGLOBULIN / B-LINEAGE DENDRITIC CELL |
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| Research Abstract |
Hodgkin's disease is a unique malignant lymphoma characterized by proliferation of Reed-Sternberg (R-S) cells and Hodgkin (H) cells, whose normal counterpart have not yet been identified, although the most recent evidence suggests a lymphoid origin.
This project aim to elucidate normal counterpart of R-S and H cells and further to analysis their biological properties to understand pathogenesis of Hodgkin's disease.
More than 21 long-term cultured cell lines derived from patients with malignant lymphoma and leukemia were investigated their immunological properties> Among them, HPL-Hod-2 (Hod-2), a lymphoblastoid cell line had been established from pleural effusion of a patient with Hodgkin's disease> Hod-2 cells shared common cell markers of R-S and H cells such as, CD15, CD25, CD30 and possessed pretty amount of IgG in their cytoplasms.
But, they lacked surface immunoglobulin expression.
Molecular analysis revealed these IgG consisted of H-chains and k-chains with almost equal molecular size to cytoplasmic IgG of ordinary B-cells.
Interestingly enough, it was found that Hod-2 cells express CD83 on their cell surface. CD83 is thought to be the most reliable makers of dendritic cells.
Among 21 human malignant lymphoma / leukemia cell lines, one hairly leukemia cell one and one B-cell malignant lymphoma cell line express weakly CD83 in their cytoplasms besides Hod-2 cells.
In conclusion ; this investigation revealed the presence of B-lineage DC and their possible original cells of H and R-S cells.
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Research Products
(6 results)
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[Publications] Nagasaki, M., Morikawa, S., Torii, I., Zhang, J., and Morikawa, K.: "A human B-lineage dendritic cell line, HBM-Noda and its potential role in GTLV-I infection"Pathol. Int.. 50 (in press). (2000)
Description
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