1999 Fiscal Year Final Research Report Summary
Morphological analysis of early stage of mycosis cell in immunologic microenvironment including apoptosis
| Project/Area Number |
10670173
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Human pathology
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| Research Institution | Saitama Medical School |
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Principal Investigator |
ARAI Eiichi Saitama Medical School, Instructor, 医学部, 講師 (60167228)
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| Project Period (FY) |
1998 – 1999
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| Keywords | PCR / In situ hybridization / T-cell receptor-β / cutaneous pseudolymohoma / cutaneous lymphoma / mycosis fungoides / pseudolymphomatous folliculitis |
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| Research Abstract |
After completing polymerase chain reaction (PCR)-in situ method for detecting lymphoma cells morphologically showing the rearrangement of T-cell receptor (TCR) β-chain gene, we attempted to use that method in some cases using.formalin-fixed paraffin-embedded materials. First, we got positive band by reverse transcriptase (RT)-PCR method (Yumoto, et al: Virchow Archiv 1995;425:11) using fresh frozen tissues of mycosis fungoides (MF). Second, we detected the rearranged TCR β-chain gene after sequencing of the positive band. Using the detected arrangement we made primers for PCR and a Dig-labbelled probe for in situ hybridization. We applied the PCR-in situ method (Nuovo: Third Ed, New York, Raven Press, 1 997) of TCR β-chain gene for some paraffin sections of biopsy of MF. As the result of that method, we detected positive dots in nuclei by staning alkaliphosphatase but showing marked morphological destruction. In the next project we will try to increase sensitivity in detective the rearranged TCR-β chain gene reserving surrounding reactive cells and microenvironment.
We reported the clinical, hidtopathologic, and immunohistologic features of pseudolymphomatous folliculitis (PLF). We concluded that PLF is a subset of cutaneous lymphoid hyperplasia with characteristic clinical and pathologic features showing perifollicular clustering of T-cell associated dendritic cells with activation of pilosebceous units.
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