1999 Fiscal Year Annual Research Report

HTLV産生細胞の各種抗原発現に関する免疫電子顕微鏡的研究

Research Project

Project/Area Number	10670209
Research Institution	Kochi Medical School
Principal Investigator	大朏祐治高知医科大学, 医学部, 教授 (30136387)
Keywords	HTLV-I / HTLV-II / T細胞株 / T細胞受容体 / CD3 / 免疫電顕 / HHV-8 / PEL
Research Abstract	HTLV-I、-II産生細胞株について、T細胞受容体(TCRα/β)、およびそのα鎖、β鎖について発現状況を検討し、特に新鮮ヒトリンパ球におけるそれらと比較検討した。まずHTLV-I、-II細胞では、CD25、HLA-DRは共に強陽性であり、CD4は、HTLV-I産生長期培養株では減弱していたが、CD3は一部で残存しており、細胞膜表面のみでなく、核膜周囲腔や粗面小胞体に陽性であった。TCRα/βは、HTLV-Iや-II産生細胞株では細胞表面には、ごく一部で認められたのみで殆ど陰性であった。TCRα鎖は、新鮮リンパ球では、核膜周囲腔や粗面小胞体にTCRβ鎖と共に陽性であったが、培養細胞では、TCRα鎖の減弱、消失やTCRβ鎖の遺残をみ、表面に陽性であったTCRα/βも陰性化した。HTLV-I、-II産生細胞では、TCRβ鎖が一部で陽性であったのみで、TCRα/β、TCRα鎖は共に陰性化していた。これらの結果は、HTLV-I、-II産生細胞では、T細胞受容体複合体(TCR・CD3)が不完全であり、成熟したT細胞としての機能を有さない機能不全状態にあることを示唆している。また、TCRα/βの細胞表面からの消失は、新鮮リンパ球でも明らかであったようにα鎖の消失に続く二次的なものであることが示唆された。またPrimary effusion lymphoma(PEL)由来のKS-1細胞とそのTPA処理細胞とで行ったHHV-8の超微形態所見では、TPA処理において核内のみでなく胞体外に放出される未熟ないし成熟粒子が多数観察され、外径250μmをこえる巨大粒子や発芽粒子も確認された。TPA未処理細胞では核内に少数の粒子をごく少数の細胞で認めたのみであった。(Ohtsuki et al,Med Electyron Microsc,1999)。これらのデータはHHV-8粒子の微細構造を明らかにした責重なデータである。

Research Products
(15 results)

All Other

All Publications (15 results)

[Publications] Y.Qiu,et al: "Application of an in situ PCR hybridization mithod to detection of human T-lymphotropic virus type I-infected cells in the lung"Acta Med Okayama. 53(1). 1-4 (1999)
[Publications] H Murakami,et al: "Detemination of the prognostic significance of cyclin B1 overexpression in patients with esophageal squamous cell carcinoma"Virchows Arch. 434. 153-158 (1999)
[Publications] Y Ueda,et al: "Expression of cytokeratin 19 mRNA in human lung cancer cell lines"Int J Cancer. 81. 939-943 (1999)
[Publications] BK Chaen,et al: "Overexpression of c-Met protein in human thyroid tumors correlated with lymph node metastasis and clinicopathologic stage"Pathol Res Pract. 195. 427-433 (1999)
[Publications] M Matsumoto,et al: "Comparison of deregulated expression of cyclin D1 and cyclin E with that of cyclin-dependent kinase 4 (CDK4) and CDK2 in human oesophageal squamous cell carcinoma"Brit J Cancer. 80(1/2). 256-261 (1999)
[Publications] SB Liang,et al: "Sun-exposure- and aging-dependent p53 protein accumulation results in growth advantage for tumour cells in carcinogenesis of nonmelanocytic skin cancer"Virchows Arch. 434. 193-199 (1999)
[Publications] BK Chaen,et al: "Co-overexpression of p53 protein and epidermal growth factor receptor in human papillary thyroid carcinomas correlated with lymph node metastasis,tumor size and clinicopathologic atage"Int J Oncol. 15. 893-898 (1999)
[Publications] J Fujita,et al: "Elevated of anti-cytokeratin 19 anitibody in sera of the patients with idiopathic pulmonary fibrosis and pulmonary fibrosis associated with collagen vascular disorders"Lung. 177. 311-319 (1999)
[Publications] Y Ohtsuki,et al: "Ultrastructure of Kaposi's sarcoma-associated herpesvirus (KSHV)/human herpesvirus-8(HHV-8) in a primary effusion lymphoma cell line treated with tetradecanyol phorbol phorbol acetate(TPA)"Med Electron Microsc. 32. 94-99 (1999)
[Publications] J Fujita,et al: "Detection of large molecular weight cytokeratin 8 as carrier protein of CA199 in non-small-cell lung cancer cell lines"Brit J Cancer. 81(5). 769-773 (1999)
[Publications] N Dobashi,et al: "Elevated serum and BAL cytokeratin 19 fragment in pulmonary fibrosis and acute interstitial pneumonia"Eur Respir J. 14. 574-578 (1999)
[Publications] TX Jin,et al: "Human mismatch repair gene (hMSH2) product expression in relation to recurrence of transitionl cell carcionma of the urinarv bladder"Cancer. 85(2). 478-484 (1999)
[Publications] J Fujita,et al: "Elevation of cytokeratin 19 fragment in patients with interstitial pneumonia associated with polymyositis/dermatomyositis"J Rheumatol. 26. 2377-2382 (1999)
[Publications] M Furihata,et al: "Missense mutation of the hMSH6 and p53 gene in sporadic urothelial transitional cell carcinoma"Int L Oncol. 16. 491-496 (2000)
[Publications] Ohtsuki,et al: "Two cases of cutaneous anaplastic large cell lymphoma,immunohistochemically Positive for CD30,HLA-DRα and IL-5 in "Lymphoreticular cells and diseases""Kowa Publishing Co,Okayama,Jpn. 437 (1999)

1999 Fiscal Year Annual Research Report

HTLV産生細胞の各種抗原発現に関する免疫電子顕微鏡的研究

Principal Investigator

大朏 祐治 高知医科大学, 医学部, 教授 (30136387)

Research Products

[Publications] Y.Qiu,et al: "Application of an in situ PCR hybridization mithod to detection of human T-lymphotropic virus type I-infected cells in the lung"Acta Med Okayama. 53(1). 1-4 (1999)

[Publications] H Murakami,et al: "Detemination of the prognostic significance of cyclin B1 overexpression in patients with esophageal squamous cell carcinoma"Virchows Arch. 434. 153-158 (1999)

[Publications] Y Ueda,et al: "Expression of cytokeratin 19 mRNA in human lung cancer cell lines"Int J Cancer. 81. 939-943 (1999)

[Publications] BK Chaen,et al: "Overexpression of c-Met protein in human thyroid tumors correlated with lymph node metastasis and clinicopathologic stage"Pathol Res Pract. 195. 427-433 (1999)

[Publications] M Matsumoto,et al: "Comparison of deregulated expression of cyclin D1 and cyclin E with that of cyclin-dependent kinase 4 (CDK4) and CDK2 in human oesophageal squamous cell carcinoma"Brit J Cancer. 80(1/2). 256-261 (1999)

[Publications] SB Liang,et al: "Sun-exposure- and aging-dependent p53 protein accumulation results in growth advantage for tumour cells in carcinogenesis of nonmelanocytic skin cancer"Virchows Arch. 434. 193-199 (1999)

[Publications] BK Chaen,et al: "Co-overexpression of p53 protein and epidermal growth factor receptor in human papillary thyroid carcinomas correlated with lymph node metastasis,tumor size and clinicopathologic atage"Int J Oncol. 15. 893-898 (1999)

[Publications] J Fujita,et al: "Elevated of anti-cytokeratin 19 anitibody in sera of the patients with idiopathic pulmonary fibrosis and pulmonary fibrosis associated with collagen vascular disorders"Lung. 177. 311-319 (1999)

[Publications] Y Ohtsuki,et al: "Ultrastructure of Kaposi's sarcoma-associated herpesvirus (KSHV)/human herpesvirus-8(HHV-8) in a primary effusion lymphoma cell line treated with tetradecanyol phorbol phorbol acetate(TPA)"Med Electron Microsc. 32. 94-99 (1999)

[Publications] J Fujita,et al: "Detection of large molecular weight cytokeratin 8 as carrier protein of CA199 in non-small-cell lung cancer cell lines"Brit J Cancer. 81(5). 769-773 (1999)

[Publications] N Dobashi,et al: "Elevated serum and BAL cytokeratin 19 fragment in pulmonary fibrosis and acute interstitial pneumonia"Eur Respir J. 14. 574-578 (1999)

[Publications] TX Jin,et al: "Human mismatch repair gene (hMSH2) product expression in relation to recurrence of transitionl cell carcionma of the urinarv bladder"Cancer. 85(2). 478-484 (1999)

[Publications] J Fujita,et al: "Elevation of cytokeratin 19 fragment in patients with interstitial pneumonia associated with polymyositis/dermatomyositis"J Rheumatol. 26. 2377-2382 (1999)

[Publications] M Furihata,et al: "Missense mutation of the hMSH6 and p53 gene in sporadic urothelial transitional cell carcinoma"Int L Oncol. 16. 491-496 (2000)

[Publications] Ohtsuki,et al: "Two cases of cutaneous anaplastic large cell lymphoma,immunohistochemically Positive for CD30,HLA-DRα and IL-5 in "Lymphoreticular cells and diseases""Kowa Publishing Co,Okayama,Jpn. 437 (1999)

大朏祐治高知医科大学, 医学部, 教授 (30136387)