1999 Fiscal Year Final Research Report Summary
A study on oostatic hormone which induces apoptosis of developing ovarian follicles in mosquitoes
| Project/Area Number |
10670238
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
寄生虫学(含医用動物学)
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| Research Institution | Juntendo University |
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Principal Investigator |
UCHIDA Keikichi Juntendo University, Department of Biology, Lecturer, 医学部, 講師 (40053368)
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| Co-Investigator(Kenkyū-buntansha) |
ESHITA Yuki Kurume University, Department of Parasitology, Lecturer, 医学部, 講師 (10082223)
OHMORI Daijirou Juntendo University, Department of Chemistry, Assistant Professor, 医学部, 助教授 (00124967)
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| Project Period (FY) |
1998 – 1999
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| Keywords | Culex pipiens pallens / ovarian development / follicular degeneration / oostatic hormone / apoptosis |
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| Research Abstract |
Oostatic hormone induces degeneration and absorption of some proportion of developing follicles (oosorption), thus to adjust the number of finally-matured follicles to the available nutrients. In order to identify this hormone in mosquitoes, we examined mosquito ovaries either by in vitro or in vivo method and have obtained the following results and information.
1) Preliminary in vitro study confirmed that mosquito ovaries may maintain its normal morphology when incubated in TC100 medium for 24〜48 h with gentle aggitation at 22〜24℃.
2) Ovarian follicles removed from newly-emerged females developed slightly when cultured in the above condition with 0.1μg JH added into the medium.
3) However, the same follicles from emerged adults did not show any retarded growth or degeneration when cultured with mature ovaries, thus suggesting the in vitro assay inadequate for oostatic hormone study.
4) In vivo study, follicles of decapitated or ligated specimens showed slightly retarded growth when culture medium of matured-ovaries was injected into the hemocoel, compared with control specimens, thus indicating this in vivo method useful for oostatic hormone assays.
We are now undertaking the above in vivo study to isolate oostatic substance(s) from matured or developing ovaries after a blood meal.
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