• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2000 Fiscal Year Final Research Report Summary

The control of bacterial infectious diseases by the orchestra of immune system and nerve system

Research Project

Project/Area Number 10670247
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Bacteriology (including Mycology)
Research InstitutionHirosaki University

Principal Investigator

NAKANE Akio  Hirosaki University School of Medicine, Professor, 医学部, 教授 (30164239)

Project Period (FY) 1998 – 2000
KeywordsListeria monocytogenes / Sympathetic nerve / Host defense / IL-12 / IFN-γ / TNF-α / Norepinephrine / Adrenergic receptor
Research Abstract

The intimate interaction between immune system and nerve system is known to be involved in homeostasis. We studied the orchestra betwwen these two systems in Listeria monocytogenes-infected mouse model in which the sympathetic system, especially noradrenergic nerve terminals, is destroyed by administration of 6-hydroxydopamine (6-OHDA).
(1) When mice was pretreated with 6-OHDA), host defense to L.monocytogenes infection was augmented late in infection. This effect was prevented by pretreatment with desipramine, which blocks the uptake of 6-OHDA into the nerve fibers.
(2) We investigated the effect of denervation by 6-OHDA on phagocytic, listericidal, and NO production by splenic macrophages and peritoneal macrophages. However, activation of either macrophages was not observed.
(3) We investigated the effect of denervation by 6-OHDA on T-cell-dependent antilisterial resistance by depletion of T-cell subsets in vivo. Augmentation of the resistance by denervation was abolished by depletion of CD8 T cells, suggesting that CD8 T cells are involved in this phenomenon.
(4) We investigated the effect of denervation by 6-OHDA on cytokine production by splenic dendritic cells. IL-12, TNF-α and IFN-γ production induce by heat killed L.monocytogenes was augmented, suggesting that IL-12-mediated induction of T-helper 1 polarization by dendritic cells might be involved in augmentation of antilisterial resistance by denervation.
(5) We also investigated the role of adrenergic receptors on host defense to L.monocytogenes infection. Administration of α-adrenergic receptor antagonists, but not β-adrenergic receptor antagonists, inhibited antilisterial resistance, suggesting that α-adrenergic receptors are involved in host defense to L.monocytogenes infection.
Our results demonstrated that the intimate interactions between immune system and nerve system might be required to control L.monocytogenes infection.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] S.Nishikawa: "Host resistance against Listeria monocytogenes is reciprocal during the course of infection in alymphoplastic aly mutant mice"Cellular Immunology. 187・2. 88-94 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] A.Nakane: "Endogenous cytokines during a lethal infection with Listeria monocytogenes in mice"FEMS Microbiology Letter. 175・1. 133-142 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.Sasaki: "Interleukin-4 and interleukin-10 are involved in host resistance to Staphylococcus aureus infection through regulation of gamma interferon"Infection and Immunity. 68・5. 2424-2430 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Miura: "Host resistance to Listeria monocytogenes infection is enhanced but resistance to Staphylococcus aureus infection is reduced in acute graft-versus-host disease"Infection and Immunity. 68・7. 4340-4343 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Miura: "Roles of endogenous cytokines in liver apoptosis of mice in lethal Listeria monocytogenes infection"FEMS Immunology and Medical Microbiology. 28・4. 1883-1888 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] D.Mizuki: "Interference between host resistance to Listeria monocytogenes infection and ovalbumin-induced allergic responses in mice"Infection and Immunity. 69・3. 335-341 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nishikawa, S., and A.Nakane.: "Host resistance against Listeria monocytogenes is reciprocal during the course of infection in alymphoplastic aly mutant mice. resistance against Listeria monocytogenes infection in mice."Cellular Immunology. 187(2). 88-94 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakane, A., K.Yamada, S.Hasegawa, D.Mizuki, M.Mizuki, S.Sasaki, and T.Miura.: "Endogenous cytokines during a lethal infection with Listeria monocytogenes in mice."FEMS Microbiology Letters. 175(1). 133-142 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sasaki, S., S.Nishikawa, T.Miura, M.Mizuki, K.Yamada, H.Madarame, and A.Nakane.: "Interleukin-4 and interleukin-10 are involved in host resistance to Staphylococcus aureus infection through regulation of gamma interferon."Infection and Immunity. 68(5). 2424-2430 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Miura, T.D.Mizuki, S.Sasaki, S.Hasegawa, H.Sashinami and A.Nakane.: "Host resistance to Listeria monocytogenes infection is enhanced but Staphylococcus aureus infection is reduced in acute graft-versus-host disease."Infection and Immunity. 68(7). 4340-4343 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Miura, T., S.Nishikawa, S.Sasaki, Y.Yamada, S.Hasegawa, D.Mizuki, M.Mizuki, I.Hatayama, K.Sekikawa, Y.Tagawa, Y.Iwakura and A.Nakane.: "Roles of endogenous cytokines in liver apoptosis of mice in lethal Listeria monocytogenes infection."FEMS Immunology and Medical Microbiology. 28(4). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mizuki, D., T.Miura, S.Sasaki, M.Mizuki, H.Madarame, and A.Nakane.: "Interference between host resistance to Listeria monocytogenes infection and ovalbumin-induced allergic responses in mice."Infection and Immunity. 69(3). 1883-1888 (2001)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 2002-03-26  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi