1999 Fiscal Year Final Research Report Summary
Molecular biology of oligoclonal ββィイD1+ィエD1 T cells in murine inflammatory bowel disease
| Project/Area Number |
10670302
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Immunology
|
|---|
| Research Institution | Osaka University |
|---|
Principal Investigator |
TAKAHASHI Ichiro Res. Inst. microbial Dis., Osaka University Associate Professor, 微生物病研究所, 講師 (20206791)
|
|---|
| Project Period (FY) |
1998 – 1999
|
| Keywords | ββ T cells / Th2 cytokine / IBD / mucosal T cells / clonality / gut-flora |
|---|
| Research Abstract |
A population of CD4ィイD1+ィエD1 T cells with TCR β-chain without TCR α-chain (CD4ィイD1+ィエD1,ββィイD1+ィエD1 T cells) producing Th2-type cytokines increased in the mucosal and peripheral tissues of TCR α-chain deficient mice with inflammatory bowel disease (IBD). Analysis of TCR-β immunoprecipitates by two-dimensional electrophoresis and RT-PCR revealed TCR of the CD4ィイD1+ィエD1 T cells was a homodimer of TCR β-chains. PCR-SSCP analyses of TCR νβ-chain transcripts of the ββィイD1+ィエD1 T cells revealed monoclonal to oligoclonal accumulation of the cells in the colon, suggestingclonal expansion of the mucosal ββィイD1+ィエD1 T cells upon the stimulation with gut-derived antigens. The homodimer of TCR β-chains on the ββィイD1+ィエD1 T cells was a biologically functional receptor which transducedactivation signals provided by MHC-class ll-associated peptidic antigens and superantigens. The importance of the Th2-biased ββィイD1+ィエD1 T cells was also supported by the finding that treatments of the mutant mice with mAb against TCR βor lL-4 suppressed the onset of IBD. The Th2-biased cytokine production by the ββィイD1+ィエD1 T cells in IBD mice could be attributed to the high incidence of Bacteroides vulgatus. Rectal administration of non-diseased mice with B. vulgatus resulted in the development of Th2-type ββィイD1+ィエD1 T cell-induced colitis. These findings suggest that the generation of oligoclonal Th2-type ββィイD1+ィエD1 T cells plays a critical role for the development of IBD.
|
Research Products
(11 results)
-
-
-
-
-
-
-
-
[Publications] Takahashi, I., H. Iijima, D. Kishi, S. Hamada, R. Katashima, M. Itakura, and H.Kiyono: "Biochemical characterization of CD4ィイD1+ィエD1, αィイD1-ィエD1βィイD1+ィエD1 T cells in TCR-α mutant mice with inflammatory bowel disease. In : Induction and modulation of gastrointestinal nflammation. M. Zeitz (eds.)"Kluwer academic publishers, Lancaster, UK. 142-146 (1998)
Description
「研究成果報告書概要(欧文)」より
-
[Publications] Boyaka, P. N., M. Marinaro, J. L. VanCott, I. Takahashi, K. Eujihashi, M. Yamamoto, F. Van Ginkel, R. J. Jackson, H. Kiyono, and J. R. McGhee: "Strategies for mucosal vaccine development"Am. J. Tropp. Med. Hyg. 60. 35-45 (1999)
Description
「研究成果報告書概要(欧文)」より
-
-
