1999 Fiscal Year Final Research Report Summary
Haplotype analysis of two STRs within the vWF gene in Japanese population
Project/Area Number |
10670384
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Legal medicine
|
Research Institution | Niigata University |
Principal Investigator |
DEWA Koji Niigata Univ School of Medicine Assistant Professor, 医学部, 助教授 (10197832)
|
Co-Investigator(Kenkyū-buntansha) |
NAGAI Atsushi Gifu Univ School of Medicine Assistant, 医学部, 助手 (00207961)
NAITO Emiko Niigata Univ School of Medicine Assistant, 医学部, 助手 (80018811)
|
Project Period (FY) |
1998 – 1999
|
Keywords | Haplotype analysis / vWF gene / STR / polymorphism information content / personal discrimination / paternity testing |
Research Abstract |
A haplotype can be more polymorphic than an allotype, and therefore its usefulness in forensic investigations has been examined. Two tetrameric repeat loci, vWF-k and vWF-p, were chosen as a haplotype probe, which are located within approximately 700 bp of intron 40 of the von Willebrand factor gene. Previously, we determined their polymorphisms by PCR for DNA obtained from a total of 57 families and the haplotypes were constructed on the basis of the family data. No significant deviations from Hardy-Weinberg equilibrium could not be found in the 116 unrelated Japanese samples. In the present study, the vWF-k/vWF-p haplotype was determined in another 26 families and the data was added to previous one. From 169 unrelated individuals included in the 83 families combined, 32 haplotypes and 97 genotypes were identified (χィイD12ィエD1 : 573, df : 496, p : 0.009). Its heterozygosity and polymorphism information content were 0.947 and 0.921, respectively. The distribution has not fitted Hardy-Weinberg equilibrium yet. However, 3 of 32 haplotypes and 20 of 97 genotypes were newly detected and the two STR haplotype showed to be highly polymorphic. This indicates the value of the haplotype analysish in personal discrimination, especially in cases where there is family information.
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Research Products
(2 results)