1999 Fiscal Year Final Research Report Summary

PR-39 gene transduction suppresses heaptocellular carcinoma cell metastasis by inhibition of signal transduction

Research Project

Project/Area Number	10670444
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Gastroenterology
Research Institution	ASAHIKAWA MEDICAL COLLEGE
Principal Investigator	FUJIMOTO Yoshinori Asahikawa Medical College, Medicine Instructor, 医学部, 助手 (90292127)
Co-Investigator(Kenkyū-buntansha)	KOHGO Yutaka Asahikawa Medical College, Medicine Professor, 医学部, 教授 (10133183)
Project Period (FY)	1998 – 1999
Keywords	antimicrobial peptide / PR-39 / syndecan / actin / signal transduction / hepatocellular carcinoma / MAP kinase
Research Abstract	PR-39 is an endogenous proline-rich antimicrobial peptide which induces the synthesis of syndecan-1, a transmembrane heparan sulfate proteoglycan involved in cell-to-matrix interactions and wound healing. Previously, we revealed that PR-39 has functions involved in the suppression of motile activity and alteration of actin structure on human hepatocellular carcinoma cells in addition to the suppression of invasive activity which might result from the induction of syndecan-1 expression. To clarify this mechanism we transfected PR-39 gene into NIH3T3 cells transformed with activated k-ras gene. The PR-39 gene transfectant revealed changes of morphology, actin structure, proliferation rate and MAPK activity. PR-39 might affect ras signal transduction.

(4 results)