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2001 Fiscal Year Final Research Report Summary

EXPRESSION AND CYTOPROTECTIVE FUNCTION OF HEAT SHOCK PROTEINS IN THE GASTRIC MUCOSA MEDIATED BY CENTRAL NERVOUS SYSTEM-RELATED NEUROPEPTIDES.

Research Project

Project/Area Number 10670445
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Gastroenterology
Research InstitutionAkita University

Principal Investigator

OTAKA Michiro  Akita University, School of Medicine Lecturer, 医学部, 講師 (30250872)

Co-Investigator(Kenkyū-buntansha) ITOH Hideaki  Akita University,.School of Medicine associate proffessor, 医学部, 助教授 (80168369)
Project Period (FY) 1998 – 2001
Keywordsheat shock protein / stress protein / gastric mucosal damage / neuro peptide / TRH / 5-HT / molecular chaperon
Research Abstract

We have studied the expression and cytoprotective function of heat shock protein (HSP) in the gastric mucosa supported by this grant. We have found that systemic stress, such as water-immersion stress, induces 72-kDa heat shock protein (HSP72) and 60-kDa heat shock protein (HSP60) in the gastric mucosa. Induction of these HSPs in the gastric mucosa enhances cytoprotective ability against cytotoxic agents including 0.6N HC1, aspirin and ethanol. Also, we found that administration of tyhrotropin releasing hormone (TRH) specifically induces gastric mucosal HSP72 expression. On the other hand, serotonin (5-HT) specifically induces gastric mucosal HSP60 expression. Using these specific induction methods, we could compare the cytoprotective ability of these HSPs. Whereas HSP72 induction in the gastric mucosa showed strong mucosal protective ability, HSP60 did not have mucosal protective ability against cytotoxic agents. Our results indicate that HSP72 could be endogenous cytoprotectant in the gastric mucosa mediated by the function of molecular chaperon. We concluded that HSP72-induction therapy might be useful for peptic ulcers or drag-induced gastric mucosal damages.

  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] Sasahara H, Otaka M, Itho H. et al.: "Effect of pre-induction of heat shock proteins on acetic acid-induced small intestinal lesion in rats"Dig Dis Sci. 43. 2117-2130 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Otaka M, Osashima M, Itho H, et al.: "Role of calcineurin, protein phosphatase 2B, in gastric acid secretion"Molecular Biological Approach to Gastrointestinal Disease. Axel Springer Japan Publishing Inc.. 13-16 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Jin M, Otaka M, Itoh H, et al.: "Associatiation of a 72-kDa heat shock protein expression with adaptation to aspirin in rat gastric mucosa"Dig Dis Sci. 44. 1401-1407 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Odashima M, Otaka M, et al.: "Effects of Cyclosporin A on water-induced gastric lesion and gastric secretion in rats"J Gastroenterol. 35. 369-371 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Iwabuchi A, Otaka M, Itoh H, et al.: "Specific pre-induction of a 60-kDa heat shock protein (chaperonin homolog) by TRH does not protect colonic mucosa against acetic acid-induced lesion in rats"Dig Dis Sci. 45. 1480-1489 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Pacheco I, Otaka M, et al.: "Corticosteroid pretreatment prevents small intestinal mucosal lesion induced by acetic acid-perfusion model in rats"Dig Dis Sci. 45. 2337-2346 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Otaka M: "Stress-related events regulate serum leptin level"Molecular Biological Approach to Gastrointestinal Disease. Axel Springer Japan Publishing Inc.. 13-16 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Pacheco I, Otaka M, et al.: "ER167653, a potent suppressant of interleukin-1 and tumor necrosis factor-α production, ameliorates colonic lesions in experimentally induced acute colitis"J Gastroenterol Hepatol. 16. 1105-1111 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Otaka M, Watanabe S.: "Role of mucosal mast cells in Helicobacter pylori infection(Editorial)"Gastroenterol. 37. 70-72 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Itoh S, Otaka M, et al.: "Inhibition of CN(protein phosphatase-2B) suppresses Ca^<2+>-mediated acid secretion in rtas"Ailment Pharmacol Ther. 16. S26-S28 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 大高道郎: "今日の治療指針 2002 急性胃炎、急性胃潰瘍"医学書院、東京. 1527(296-297) (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sasahara H, Otaka M, Itho H, et al.: "Effect of pre-induction of heat shock proteins acetic acid-induced small intestinal lesion in rats"Dig Dis Sci. 43. 2117-2130 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Otaka M, Odashima M, Itho S.: "Role of calcineurin, protein phosphatase 2B, in gastric acid secretion"Molecular Biological Approach to Gastrointestinal Disease. Axel Springer Japan Publishing Inc.. 13-16 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Jin M, Otaka M, Itoh H. et al.: "Association of a 72-kDa heat shock protein expression with adaptation to aspirin in rat gastric mucosa"Dig Dis Sci. 44. 1401-1407 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Odashima M, Otaka M, et al.: "Effects of Cyclosporin A on water-immersion stress-induced gastric lesion and gastric secretion inrats"J Gastroenterol. 35. 369-371 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Iwabuchi A, Otaka M, Itoh H, et al.: "Specific pre-induction of a 60-kDa heat shock protein (cnaperonin homolog) by TRH does not protect colonic mucosa against acetic acid-induced lesion in rats"Dig Dis Sci. 45. 1480-1489 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Pacheco I, Otaka M et al.: "Corticosteroid pretreatment prevents small intestinal mucosal lesion induced by acetic acidperfusion model in rats"Dig Dis Sci. 45. 2337-2346 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Otaka M.: "stress-related events regulate serum leptin level"Molecular Biological Approach to Gastrointestinal Disease. Axel Springer Japan Publishing Inc.. 13-16 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Pacheco I, Otaka M, et al.: "FR167653, a potent suppressant of interleukin-1 and tumor necrosis factor-aproduction, ameliorates colonic lesions in experimentally induced acute colitis"J Gastroenterol Hepatol. 16. 1105-1111 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Otaka M, Watanabe S.: "Role of mucosal mast cells in Helicobacter pylori infection. (Editorial)"J Gastroenterol. 37. 70-72 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Itoh S. Otaka M, et al.: "Inhibition of CN(protein phosphatase-2B) suppresses Ca2+-mediated acid secretion in rats"Aliment Pharmacol Ther. 16. S26-28 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Otaka M.: "Acute gastritis, Acute gastric ulcer"Today's therapy 2002. Igakusyoin, Tokyo. 1527(in Japanese) (2002)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2003-09-17  

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