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1999 Fiscal Year Final Research Report Summary

Analysis of the pathogenesis of inflammatory bowel disease using experimental colitis induced by a murine retrovirus

Research Project

Project/Area Number 10670457
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Gastroenterology
Research InstitutionNIIGATA UNIVERSITY

Principal Investigator

NARISAWA Rintaro  School of Medicine, NIIGATA UNIVERSITY Lecturer, 医学部, 講師 (30180540)

Co-Investigator(Kenkyū-buntansha) SUZUKI Kenji  School of Medicine, NIIGATA UNIVERSITY Assistant, 医学部, 助手 (00303123)
ASAKURA Hitoshi  School of Medicine, NIIGATA UNIVERSITY Professor, 医学部, 教授 (20051451)
Project Period (FY) 1998 – 1999
KeywordsUlcerative colitis / Experimental colitis / Murine AIDS / Murine leukemia virus LP-BM5 / Sjoegren's syndrome / Macrophage / Cytokine
Research Abstract

BACKGROUND : LP-BM5 murine leukemia virus (MuLV) is known to induce murine AIDS (MAIDS). We have shown that sjogren's syndrome (SjS) -like exocrinopathy can be induced in mice with MAIDS and that adoptive transfer of spleen cells from MAIDS mice can induce inflammatory bowel disease-like colitis as well as SjS-like exocrinopathy in nude mice. AIM : To assess the role of interferon (IFN)-g and interleukin (IL)-10 in the pathogenesis of our experimental model, we tried to identify the cells producing these cytokines and their localization in the colitis lesions in situ. METHODS : Four-week-old C57BL/6 (B6) mice were inoculated intraperitoneally with LP-BM5 MuLV.Eight weeks after infection, lymph node cells prepared from the virus-infected mice were transferred to B6 nude mice. Four to six weeks after the cell transfer, mice with clinical symptoms such as diarrhea, anal bleeding and body weight loss were killed to examine their colons. Expression of mRNA for IFN-g and IL-10 was assessed b … More y RTPCR, and protein expression of these cytokines was also analyzed in frozen sections of colon by double color-staining immunofluorescence (IF). RESULTS : All of the mice inoculated with MAIDS lymph node cells showed colitis as well as systemic exocrinopathy, and died within 6 weeks after cell transfer. Scattered erosions of the colon were observed. CD4+ cells were dominant and distributed diffusely within the mucosal and submucosal layers. A few CD8+ cells were detected among the intraepithelial lymphocytes of the colon. Mac-1+ cells were usually localized diffusely within the lamina propria and characteristically detected beneath the injured epithelial cells. B220+cells were localized within lymph follicles. An increase of IFN- g and IL-10 mRNA was detected in the colon of mice with colitis, but not in that of control mice. Double color IF showed that Mac-1+ cells were positive for IFN-g or IL-10 and that most CD4+ cells were positive for IL-10, although the population of IFN-g-positive CD4+ cells was low.
CONCLUSIONS : In our experimental colitis model, Mac-1+ macrophages which produce both IFN-g and IL-10 might play a crucial role in the pathogenesis of colitis in combination with CD4+ T cells. Less

  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] H Suriki et.al.: "Analysis of cytokineproduction in the colon of nude mice with experimental colitis induced by adoptive transfer of immunocompetent cells from mice infected with a murine retrovirus"Clinical Immunology. 97. 33-42 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 摺木陽久 ら: "レトロウイルス感染マウス脾細胞のヌードマウスへの移入による実験性腸炎-大腸組織における浸潤細胞の種類と分布についての解析"消化器と免疫. 35. 99-102 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 佐々木俊哉 ら: "マウスレトロウイルスにより惹起されるシエーグレン症候群様外分泌腺症におけるサイトカイン発現の検討"消化器と免疫. 35. 141-144 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 摺木陽久 ら: "マウスレトロウイルスを用いた実験性腸炎の大腸における浸潤細胞とサイトカインの関係についての解析"消化器と免疫. 36. 73-76 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 馬場靖幸 ら: "マウスレトロウイルスによる実験性腸炎における5-ASA(Mesalazine)の治療効果およびその作用機序の解析"消化器と免疫. 36. 78-81 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Suzoki et.al.: "Gastroenterology and hepatology"H.Asakura (in press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hidehisa Suriki et al.: "Analysis of cytokine production in the colon of nude mice with experimenatal colitis induced by adoptive transfer of immunocompetent cells from mice infected with a murine retrovirus"Clinical Immunology. 97. 33-42 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kenji Suzuki et al.: "Induction of colitis and exocrinopathy in nude mice induced by immunocompetent cell transfer from murine retrovirus-infected mice In Gastroenterology and hepatology, Ed by H Asakura"Springer-Verlag, Tokyo, (in press). (2001)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2002-03-26  

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