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1999 Fiscal Year Final Research Report Summary

Molecular Biological Analysis of Helicobacter pylori pathogenicity and gastric mucosa

Research Project

Project/Area Number 10670498
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Gastroenterology
Research InstitutionDokkyo University School of Medicine

Principal Investigator

SHIMADA Tadahito  Dokkyo University, School of Medicine, Dep. Of Medicine, Associate Professor, 医学部, 助教授 (10206170)

Co-Investigator(Kenkyū-buntansha) OHTSUKA Yukio  Dokkyo University, School of Medicine, Dep. Of Medicine, Instructor, 医学部, 助手 (40296140)
TERANO Akira  Dokkyo University, School of Medicine, Dep. Of Medicine, Professor, 医学部, 教授 (50155470)
HIRAISHI Hideyuki  Dokkyo University, School of Medicine, Dep. Of Medicine, Associate Professor, 医学部, 助教授 (00199035)
Project Period (FY) 1998 – 1999
KeywordsHeicohacter pylori / Gastric mucosa / Carcinogenesis / inflammation / 活性酸素
Research Abstract

Helicobacter pylori (H. pylon) is closely related to the pathogenesis of chronic gastritis, peptic ulcer disease, gastric carcinoma, and gastric MALT-lymphoma. However, the mechanisms of H. Pylon-induced gastric mucosal damage and carcinogenesis have not been fully understood. We investigated the effects of H. pylon infection and H. Pylon-induced gastric inflammation on the gene expression of gastric epithelial cells. Because reactive oxygen intermediates (ROI) are increased in the H. pyloni-infected gastric mucosa and ROI may be important in inducing gastric mucosal damage, we examined the gene expression profile of gastric epithelial cells in response to oxidative stress. We found that oxidative stress activates the transcription factors, NF-κB and AP-1, and upregulates the expression of interleukin-8 and cyclooxygenase-2. The expression of several genes involving cell cycle regulation, apoptosis, and MAP kinase pathways were also found to be affected by oxidative stress. These results suggest that, in H. pylon-infected gastric mucosa, prolonged inflammatory condition alters the gene expression patterns of gastric epithelial cells which may be related to the gastric carcinogenesis.

  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] T. Shimada et al: "Redox regulation of IL-8 expression in MKN28 cells"Dig, Dis. Sci.. 44. 266-273 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H. Hiraishi et al: "Palaprezinc protects gastric mucosal cells from noxicious agent through antioxidart properlies in vitro"Aliment. Pharmacol. Ther.. 13. 261-269 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H. Hiraishi et al: "Role of antioxidant defense against ethanol-induced damage in cultured rat gastric epithelial cells"J. Pharmacol. Exp. Ther.. 289. 103-109 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T. Shimada et al: "Palaprezinc down-regulates proinflammatory cytokine-induced nuclear factor-kb activation and interleukin-8 cells"J. Pharmacol. Exp. Ther.. 291. 345-352 (1999)

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      「研究成果報告書概要(和文)」より
  • [Publications] 島田忠人: "Helicobacter pylori除菌後の諸問題"Medical Practice. 16. 807-809 (1999)

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      「研究成果報告書概要(和文)」より
  • [Publications] K.Sakata, T.Shimada, H.Hiraishi, A.Terano.: "Role of cyclooxygenase-2 in hepatocyte growth factor-mediated gastric epithelial restitution"J.Clin.Gastroenterol.. 27(Suppl.1). S41-S47 (1998)

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      「研究成果報告書概要(欧文)」より
  • [Publications] N.Yamaguchi, N.Yajima, M.Ishida, T.Shimada, H.Hiraishi: "Protection of cultured gastric cells against tert-butyl hydroperoxide by glutathione isopropyl ester"Eur. J. Pharmacol.. 351. 363-369 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Shimada, A.Terano.: "Chemokine expression in Helicobacter pylori-infected gastric mucosa."J.Gastroenterol.. 33. 613-617 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Shimada,N. Watanabe, H. Hiraishi, A. Terano.: "Redox regulation of interleukin-8 expression in MKN28 cells."Dig. Dis. Sci.. 44. 266-273 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Shimada.: "Hepatocyte growth factor protects gastric epithelial cells against ceramide-induced apoptosis through induction of cyclooxygenase 2."Proceedings of the GI Forum 1998 in New Orleans Molecular Biological Approach to Gastrointestinal Disease. 20-23 (1999)

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      「研究成果報告書概要(欧文)」より
  • [Publications] H. Hiraishi, T. Sasai, T.Oinuma, T.Shimada, H.Sugaya, A. Terano.: "Polaprezinc protects gastric mucosal cells from noxious agents through antioxidant properties in vitro."Aliment. Pharmacol. Ther.. 13. 261-269 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H. Hiraishi, T. Shimada, K..J.Ivey, A. Terano.: "Role of antioxidant defense against ethanol-induced damage in cultured rat gastric epithelial cells."J. Pharmacol. Exp. Ther.. 289. 103-109 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] N. Yajima, H. Hiraishi, N. Yamaguchi, M. Ishii, T. Shimada, A. Terano: "Monoclamine-induced cytolysis to cultured rat gastric mucosal cells: Role of glutathione and iron in protection and injury."J. Lab. Clin. Med.. 134. 372-377 (1999)

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      「研究成果報告書概要(欧文)」より
  • [Publications] T. Shimada, Naomi Watanabe, Yukio Ohtsuka, Motoya Endoh, Kazuo Kojima, Hideyuki Hiraishi, Akira Terano.: "Polaprezinc down-regulates proinflammatory cytokine-induced nuclear factor-KB activation and lnterleukin-8 expression in gastric epithelial cells."J. Pharmacol Exp. Ther.. 291. 345-352 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H. Hiraishi, T. Konishi, S. Ota, T. Shimada, A. Terano, Tsuneaki Sugimoto: "Massive gastrointestinal hemorrhage in systemic lupus erythematosus: Successful treatment with corticosteroid pulse therapy."Am. J. Gastroenterol.. 94. 3349-3353 (1999)

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      「研究成果報告書概要(欧文)」より

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Published: 2001-10-23  

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