1999 Fiscal Year Final Research Report Summary
The role of epimorphin, as a morphogen, in human liver disease
| Project/Area Number |
10670506
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Juntendo University |
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Principal Investigator |
HIROSE Miyoko Juntendo University, Assistant Professor, 医学部, 助手 (70266039)
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| Co-Investigator(Kenkyū-buntansha) |
WATANABE Sumio Akita University, Professor, 医学部, 教授 (20138225)
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| Project Period (FY) |
1998 – 1999
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| Keywords | epimorphin / Hepatic sinusoid / liver regeneration / morphogenesis / liver cirrhosis |
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| Research Abstract |
To clarify the movement and role of epimorphin in human liver disease, we examined the expression of epimorphin in human liver and the effect of some growth factors on the expression of epimorphin using cultured human stellate cells,
1) Liver specimens from surgical resection or biopsy (normal, liver cirrhosis (LC)) were stained with anti-epimorphin antibody and were observed using confocal laser scanning microscopy. The exprcssion of epimorphin mRNA in human liver was observed with RT-PCR method. Epimorphin protein was detected on stellate cells in sinusoid and was stained strongly along fibrotic area of cirrhotic liver. However, the expression of mRNA in LC was almost same as in normal liver,
2) Human hepatic stellate cell line, LI90, was cultured with TGF-β, bFGF or PDGFbb. Epimorphin mRNA was extracted from treated cells and the expression of mRNA was determined by RT-PCR method. Epimorphin mRNA in human stellate cell line was induced by TGF-β, but not by bFGF and PDGFbb.
Human epimorphn was detected in stellate cells in the liver. The expression of epimorphin mRNA was induced by TGF-βwhich was secreted in the early stage of the liver damage from inflammatory cells and mesenchymal cells. Therefore, epimorphin may play an important role in the liver regeneration and morphogenesis after liver injury.
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