1999 Fiscal Year Final Research Report Summary
The Biological Functions of the SART-1 Family in Differentiation and Carcinogenicity of Adenocarcinomas
| Project/Area Number |
10670521
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Kurume University |
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Principal Investigator |
SHICHIJO Shigeki Kurume Univ., Sch. Med., Dep. Immunol., Associate Prof., 医学部, 助教授 (30080592)
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| Co-Investigator(Kenkyū-buntansha) |
ITOH Kyogo Kurume Univ., Sch. Med., Dep. Immunol., Prof., 医学部, 教授 (50125499)
IMAI Yasuhisa Kurume Univ., Sch. Med., Department of lmmunol., Assistant, 医学部, 助手 (90268847)
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| Project Period (FY) |
1998 – 1999
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| Keywords | Tumor rejection antigen / DNA-binding protein / Cell cycle / Apoptosis / Two-hybrid / ファーウエスタン / 遺伝子ファミリー / ゲノムDNA |
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| Research Abstract |
Molecules involved in regulation of cellular proliferation at the G2/M phase have not yet been fully identified. This study investigated the involvement of a recently identified SART-1ィイD2800ィエD2. Protein demonstrated an ability to bind to DNA, and accumulated in the nucleus when the cells were arrested at G2/M phase.
(l) The localization of SART-1 protein was suggested to be in nucleus of eukaryote cells including monkey kidney EBV-transformed Cos 7 cell and human esophageal cancer TE9 cell when the fusion gene with GFP was transfected and observed by conforcal laser microscopy.
(2) SART-1ィイD2800ィエD2 protein is suggested to be a DNA-binding protein by the affinity chromatography of cell lysates of the 293T cells transfected with the HAN/SART-1ィイD2800ィエD2 gene.
(3) Transfection of SART-1 gene induced the G2/M-arrest and suppressed the cell growth of all the cell tested. Furthermore, DNA ladder formation was observed in the SART-1 transfected COS-7 and human cancer cells. The transfection of SART-1 up-regulated the nuclear expression of cyclin B and cdc2, well known components of maturation-promoting factor.
(4) Several candidate genes which encode proteins that had interacted with SART-1 protein were isolated by two-hybrid system.
(5) Three different SART-1 probe protein (N-terminal, middle and C-terminal regions) for far-western blot analysis of SART-1 binding protein candidate obtained by two hybrid system were prepared.
(6) We found that murine SART-1 genomic DNA suggested to be consist of 20 exsons. The sequence of the gene will submit to GenBank.
(7) SART-1 gene was suggested to be expressed in early development of mouse embryo (l4 days) by northern blot analysis.
(8) Several family genes of SART-1 were suggested by northern blot analysis. One of the gene with 3.3kb consist of approximately l488 bp of 5'-region was identical to SART-1 and encoded a protein with 483 amino acid.
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Research Products
(19 results)
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[Publications] Yang D, Nakao M, Shichijo S, Sasatomi T, Takasu H, Matsumoto H, Mori K, Hayashi A, Yamana H, Shirouzu K, and Itoh K: "Identification of a gene coding for a protein possessing shared tumor epitopes capable of inducing HLA-A24-restricted cytotoxic T lymphocytes in cancer patients."Cancer Res. 59. 4056-4063 (1999)
Description
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[Publications] Nakao M, Shichijo S, Imaizumi T, Inoue Y, Matsunaga K, Yamada A, Kikuchi M, Tsuda N, Ohta K, Takamori S, Yamana H, Fujita H and Itoh K: "Identification of a gene coding for a new squamous cell carcinoma antigen recognized by the CTL."J. Immunol.. 164. 2565-2574 (2000)
Description
「研究成果報告書概要(欧文)」より
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