1999 Fiscal Year Final Research Report Summary
Contractility of fibroblasts derived from long fibrosis
| Project/Area Number |
10670543
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
MIO Tadashi Kyoto University Hospital, Pulmonary Medicine, Assistant Professor, 医学研究科, 助手 (90243097)
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| Co-Investigator(Kenkyū-buntansha) |
KITAICHI Masanori Kyoto University Hospital, Pathology Associate Professor, 医学研究科, 助教授 (00161464)
NAGAI Sonoko Kyoto University Hospital Pulmonary Medicine, Associate Professor, 医学研究科, 助教授 (30217955)
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| Project Period (FY) |
1998 – 1999
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| Keywords | Pulmonary Fibrosis / IPF / NSIP / Fibroblast / Contraction / IGF-β / Fibronectin |
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| Research Abstract |
The aim of this study was to compare the function of lung fibroblasts obtained from surgically biopsied specimens of patients with idiopathic pulmonary fibrosis/usual interstitial pneumonia (UIP ; n=5), nonspecific interstitial pneumonia (NSIP ; n=5), and normal parts of surgically resected lungs (control ; n=5). The results indicated that (1) fibroblasts obtained from UIP showed increased contractility compared with those obtained from NSIP or controls (UIP, 72.7±6.21% ; NSIP, 32.8±5.46 ; controls, 28.5±3.51, p<0.01 in UIP versus NSIP or control) ; (2) this increase in contractility was consistent with enhanced F-actin content in fibroblasts ; (3) conditioned media from UIP fibroblast cultures enhanced control fibroblast contractility, whereas those obtained from NSIP or controls did not ; (4) the 180 and 25 kD products representing the contractility in conditioned media were identified as fibronectin (ED-A domain) and TGF-β_1 by immunoblots, respectively ; (5) the UIP-conditioned media contained higher amounts of fibronectin or TGF-β_1(fibronectin : UIP 289±47.1 ng/ml, NSIP 121±23.0, control 118±16.0 ; TGF-β_1 : UIP 798±119 pg/ml, NSIP 246±69.1, control 247±53.6, p<0.01 in UIP versus NSIP or control) ; (5)the contractility positively correlated with the amount of either fibronectin (r=0.867, p<0.001, n=15) or TGF-β_1(r=0.939, p<0.001, n=15), respectively. Thus, UIP fibroblasts showed greater contractility than did NSIP fibroblasts and upregulated control fibroblasts.
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