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2000 Fiscal Year Final Research Report Summary

A STUDY OF TYPE II ALVEOLAR EPITHELIAL CELLS AS INFECTION SITE OF ACID-FAST BACILLI

Research Project

Project/Area Number 10670545
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Respiratory organ internal medicine
Research InstitutionSHIMANE MEDICAL UNIVERSITY

Principal Investigator

SATO Katsumasa  SHIMANE MEDICAL UNIVERSITY, MICROBIOLOGY AND IMMUNOLOGY, INSTRUCTOR, 医学部, 助手 (00142331)

Co-Investigator(Kenkyū-buntansha) SHIMIZU Toshiaki  SHIMANE MEDICAL UNIVERSITY, MICROBIOLOGY AND IMMUNOLOGY, INSTRUCTOR, 医学部, 助手 (60284030)
TOMIOKA Haruaki  SHIMANE MEDICAL UNIVERSITY, MICROBIOLOGY AND IMMUNOLOGY, PROFESSOR, 医学部, 教授 (40034045)
Project Period (FY) 1998 – 2000
KeywordsACID-FAST BACIL II / MYCOBACTERIUM TUBERCULOSIS / MYCOBACTERIUM AVIUM COMPLEX / TYPE II ALVEOLAR EPITHELIAL CELLS / MACROPHAGES / A-549 CELLS / INVASION / CYTOKINES
Research Abstract

1. Antimicrobial effects of clarithromycin (CAM), KRM-1648 (KRM) and levofloxacin (LVFX) against Mycobacterium tuberculosis (MTB) or M.avium complex (MAC) residing in the A-549 human type II alveolar epithelial cells (A-549 cells) were less than the effects of the same drugs against the same organisms residing in Mono Mac 6 human macrophage-like cells (MM6-MΦ).
2. Antimicrobial activities of these drugs against MTB and MAC adapted to an intramacrophagic environment (intracellularly adapted : I-type) and those passaged in the 7H9 liquid medium (extracellularly adapted : E-type) were studied. The antibacterial effect of CAM or LVFX against E-type MTB or MAC residing in MM6-MΦs or A-549 cells was stronerg than a case against I-type MTB or MAC within the same cells. On the other hand, the antibacterial effect of KRM for E-type MTB or MAC residing in both cells was weaker than a case for I-type organisms within the same cells.
3. Mice were infected intratracheally with MTB or MAC, and the lung sections of the infected mice were observed with transmission electron photomicrographs. The infected bacteria were recognized in type II alveolar epithelial cells in addition to mature granulocytes and macrophages.
4. Using a dual chamber system, we examined whether the humoral factor of the A-549 cells infected with MTB or MAC which were released, influenced the anti-MTB or the anti-MAC antibacterial activity of MM6-MΦs. The number of the MTB or MAC organisms residing in MM6-MΦs of the top-chamber was decreased by the existence of A-549 cells infected with the MTB or MAC.
5. It was found that the humoral factor (s) that decreased the number of these bacteria might be TNF-α and/or GM-CSF with RT-PCR examination.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Katsumasa Sato: "Antimicrobial activities of benzoxazinorifamycin KRM-1648, clarithromycin and levofloxacin against intracellular Mycobacterium avium complex phagocytosed by murine peritoneal macrophages"Journal of Antimicrobial Chemotherapy. 41. 77-83 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 佐藤勝昌: "結核治療における新薬の展望.キノロン,リファマイシン,マクロライド剤のin vitro抗結核菌および抗MAC抗菌活性,特にin vivo治療効果との関連から"結核. 74. 63-70 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Katsumasa Sato: "Antimicrobial activities of benzoxazinorifamycin (KRM-1648) and clarithromycin against Mycobacterium avium-intracellulare complex within murine peritoneal macrophages, human macrophage-like cells and human alveolar epithelial cells"Journal of Antimicrobial Chemotherapy. 43. 351-357 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 佐藤勝昌: "肺胞上皮細胞およびマクロファージ内での結核菌とMycobacterium avium complexの増殖能について"結核. 74. 655-660 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Katsumasa Sato: "Antimicrobial activities of levofloxacin, clarithromycin, and KRM-1648 against Mycobacterium tuberculosis and Mycobacterium avium complex replicating within Mono Mac 6 human macrophage and A-549 type II alveolar cell lines"International Journal of Antimicrobial Agents. 16. 25-29 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 佐藤勝昌: "抗酸菌のマクロファージ(MΦ)と肺胞上皮細胞への感染性と細胞内増殖性:MΦ内順化菌を用いての検討"結核. 76. 53-57 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Katsumasa Sato: "Antimicrobial activities of benzoxazino-rifamycin KRM-1648, clarithromycin and levofloxacin against intracellular Mycobacterium avium complex phagocytosed by murine peritoneal macrophages."Journal of Antimicrobial Chemotherapy. 41. 77-83 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Katsumasa Sato: "Prospects for development of new anti-microbials for clinical control of tuberculosis. In vitro antimicrobial activities of quinolones, refamycins and macrolides against Mycobacterium tuberculosis and M.avium complex : Attempt to establish new assay methods which accurately reflect therapeutic effects of test agents in vivo"Kekkaku. 74. 63-70 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Katsumasa Sato: "Antimicrobial activities of benzoxazinorifamycin (KRM-1648) and clarithromycin against Mycobacterium avium-intracellulare complex within murine peritoneal macrophages, human macro-phage-like cells and human alveolar epithelial cells Chemotherapy"Journal of Antimicrobial. 43. 351-357 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Katsumasa Sato: "Internalization and replication of Mycobacterium tuberculosis and M.avium complex within type II alveolar epithelial cell line"Kekkaku. 74. 655-660 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Katsumasa Sato: "Antimicrobial activities of levofloxacin, clarithromycin, and KRM-1648 against Mycobacterium tuberculosis and Mycobacterium avium complex replicating within Mono Mac 6 human macrophage and A-549 type II alveolar cell lines"International Journal of Antimicrobial Agents. 16. 25-29 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Katsumasa Sato: "Invasion and intracellular growth of Mycobacterium tuberculosis and Mycobacterium avium complex adapted to intramacrophagic environment within macrophages and type II alveolar epithelial cells"Kekkaku. 76. 53-57 (2001)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2002-03-26  

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