1999 Fiscal Year Final Research Report Summary
Molecular biological analysis of the resistant mechanism for the antifungal agents and trail of new antifungal agent
| Project/Area Number |
10670551
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | Nagasaki University |
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Principal Investigator |
MAESAKI Shigehumi Nagasaki University, School of Medicine, Assistant, 医学部, 助手 (20295059)
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| Co-Investigator(Kenkyū-buntansha) |
KOHNO Shigeru Nagasaki University, School of Medicine, Professor, 医学部, 教授 (80136647)
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| Project Period (FY) |
1998 – 1999
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| Keywords | antifungal agents / resistance / resistant gene / drug efflux |
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| Research Abstract |
We investigated the drug efflux mechanism in azole-resistant strains of Candida. Albicans using rhodamine 6G (R6G). No significant differences in R6G uptake were present between azole-sensitive B2630 (9.02±0.02 nmoL/108 cells) and azole-resistant B67081 (8.86±0.03 nmoL/108 cells) strains incubated in glucose-free phosphate buffered saline. A significantly higher R6G efflux (2.0±0.21 nmoL/108 cells) was noted in azole-resistant strain (B67081) when glucose was added, compared with that in the sensitive strain B2630 (0.23±0.14 nmoL/108 cells). A fluconazole resistant strain C40 that expressed the benomyl resistance gene (CaMDR) also showed a low R6G efflux (0.16±0.06 nmoL/108 cells) as did the sensitive strains. Accumulation of R6G in growing C. albicans cells was inversely correlated with the level of CDR1 mRNA expression. Our data also suggest that measurement of intracellular accumulation of R6G is a useful method for identification of azole-re National Committee for Clinical Laboratory Standards. (1997) Reference method for broth dilution antifungal susceptibility testing of yeasts; Approved standard M27-A. Villanova, Pennsylvania: National Committee for Clinical Laboratory Standards.
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