1999 Fiscal Year Final Research Report Summary
神経免疫疾患における新規内在性レトロウイルスの検出とその病因的意義に関する研究
| Project/Area Number |
10670605
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neurology
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| Research Institution | TOKYO WOMEN'S MEDICAL UNIVERSITY |
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Principal Investigator |
HASHIMOTO Shiroshi TOKYO WOMEN'S MEDICAL UNIVERSITY, NEUROLOGICAL INSTITUTE, INSTRUCTOR, 医学部・神経内科, 助手 (60180824)
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| Project Period (FY) |
1998 – 1999
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| Keywords | multiple sclerosis / retrovirus / MSRV / autoimmune disease |
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| Research Abstract |
Multiple sclerosis is an autoimmune disease affecting primarily the central nervous system, whose etiologies has remained unknow. Perron et al have recently identified a novel human endogenous retrovirus (MSRV) in patients with multiple sclerosis (MS). We examined whether MSRV RNA can also be detected in Japanese MS patients. RNA was isolated from plasma of Japanese MS patients. After treatment with DNase I, RNA samples were used for RT-PCR which amplifes the pol region of MSRV. Genomic DNA, which was isolated from neutrophils, was used for PCR as control. PCR products were run on an agarose gel, transferred by Southern blotting and hybridized with the probe of MSRV pol region. The pol region of MSRV was amplified from genomic DNA by PCR from all the individuals tested. On the other hand, we could not amplify the MSRV sequence by RT-PCR, from plasma RNA samplesin in MS patients. Further studies are necessary to clarify the role of MSRV in the pathogenesis of multiple seclerosis in Japanese populations.
On the other hand, during this study period, we revealed that the polymorphism at position 247 of the beta2-glycoprotein I gene is important for the generation of anti-beta2-glycoprotein I antibodies in the antiphospholipid syndrome, and that chronic lymphocytic leukemia B cells express restricted sets of mutated and unmutated antigen receptors. These findings obtained in this study would be useful for better understanding of the pathogenesis of autoimmune diseases, including multiple sclerosis.
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[Publications] Hirose N, Williams R, Alberts AR, Furie RA, Chartash EK, Jain RI, Sison C, Lahita RG, Merrill JT, Cucurull E, Gharavi AE, Sammaritano LR, Salmon JE, Hashimoto S, Sawada T, Chu CC, Gregersen PK, Chiorazzi N.: "A role for the polymorphism at position 247 of the beta2-glycoprotein I gene in the generation of anti-beta2-glycoprotein I antibodies in the antiphospholipid syndrome."Arthritis Rhuem. 42(8). 1655-1661 (1999)
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「研究成果報告書概要(欧文)」より
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[Publications] Fais F, Ghiotto F, Hashimoto S, Sellars B, Valetto A, Allen SL, Schulman P, Vinciguerra VP, Rai K, Rassenti LZ, Kipps TJ, Dighiero G, Schroeder HWJr, Ferrarine M, Chiorazzi N,: "Chronic lymphocytic leukemiaB cells express restricted sets of mutated and unmutated antigen receptors."J Clin Inest. 102(8). 1515-1525 (1998)
Description
「研究成果報告書概要(欧文)」より
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