1999 Fiscal Year Final Research Report Summary
Mdecular cloning and analysis of S' -flanking region of human MYPT1 gene
| Project/Area Number |
10670645
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Circulatory organs internal medicine
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| Research Institution | Mie University |
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Principal Investigator |
IRO Masaaki Mie University, Hospital, Lecture, 医学部・附属病院, 講師 (00223181)
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| Project Period (FY) |
1998 – 1999
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| Keywords | Myosin Phosphatase / MYPT1 / Promoter / Sp1 / Human Gene |
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| Research Abstract |
MYPT1, a subunit of myosin phosphatase, has critical functions including activation and regulation of phosphatase activity.
We have cloned and sequenced a 5 kb genomic fragment in the 5'-flanking region of the human MYPT1 gene. The transcription initiation site (+1) was 268 bp upstream from the translation start site. In this promoter there are no canonical TATA or CAAT box elements but there is a high GC-rich sequence (-58 to -64). Basal promoter activity was due to the GC-rich region that contained one Sp 1 transcription factor-binding site. The specific interaction of Sp 1 with this region was demonstrated by the electrophoresis mobility shift assay. Therefore, it was concluded that the MYPT1 gene is a housekeeping gene. Luciferase reporter assays showed the presence of two regions for positive elements (-246 to -230 and -170 to -198) and one for a negative element (-199 to -229).
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Research Products
(24 results)
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[Publications] Masaki Fujioka, Nobuaki Takahashi, Hideharu Odai, Shigemasa Araki, Kazuhito Ichikawa, Jianhua Feng, Mamoo Nakamura, Kozo Kaibuchi, David J.Hartshorne, Takeshi Nakano and Masaaki Ito: "A new isoform of human myosin phosphatase targeting/regulatory subunit (MYPT2) : cDNA cloning, tissur expression, and chromosomal mapping."Genomics 49. 59-68 (1998)
Description
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