2001 Fiscal Year Final Research Report Summary
Control of hematopoietic progenitors and molecular mechanism Influencing cell cycle
| Project/Area Number |
10670744
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Yokohama City University |
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Principal Investigator |
IKUTA Koichiro Yokohama City University, Dept of Transfusion, associate Professor, 医学部・附属病院, 講師 (80159590)
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| Co-Investigator(Kenkyū-buntansha) |
SASAKI Hideki Yokohama City University, Dept of Pediatrics, assistant Professor, 医学部, 助教授 (50106316)
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| Project Period (FY) |
1998 – 2000
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| Keywords | HEMATOPOIETIC PROGENITORS / HIGH PROLIFERATIVE POTENTIAL COLONY FORMING CELLS / SERUM-FREE CULTURE / thrombopoietin |
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| Research Abstract |
The aim of the present study was to determine the effect of thrombopoietin(Tpo) in combination with other cytokines on the growth of murine megakaryocytic, granulocyte-macrophage, erythroid and primitive hematopoietic progenitor cells, excluding possibilities of synergistic effects by serum factor(s).
Serum-free culture enabled magakaryocyte colony growth by recombinant murine(rm) Tpo and this was synergistically supported with rmIL-3, rmSCF or rhEpo. Recombinant human IL-6, rhIL-11 and rmGM-CSF showed synergistic effects with rmTPO only In the presence of serum. RmIL-3 and rm SCF Incresed the large colonies and mixed-type colonies containing other populations, suggesting that these cytokines promoted the proliferation of Immature populations of CFU-Mk. The rmTpo enhanced the growth of GM-colonies stimulated by rmGM-CSF or rmIL3 and erythroid bursts by rhEpo, with or without rmIL-3. The rmTpo also significantly incresed HPP colonies in synergy with rmIL-3 only in the presence of serum or rmSCF.
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