1999 Fiscal Year Final Research Report Summary
Molecular epidemiological study of Haemophilus influenza serotype b strains of tained from children with meningitis.
| Project/Area Number |
10670745
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Yokohama City University |
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Principal Investigator |
MITSUDA Toshihiro Yokohama City University School of Medicine, Division of clinical Laboratory Medicine, Lecture, 医学部・付属病院・臨床検査部, 講師 (00264672)
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| Project Period (FY) |
1998 – 1999
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| Keywords | Haemophilus influenzae / serotype b / RFLP / Epidemiology / meningitis / child |
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| Research Abstract |
We report an epidemiological study of 30 Haemophilus influenzae serotype b (Hib) strains derived from the cerebrospinal fluid of children with meningitis. The Hib strains were biotyped, tested for β-lactamase production, and genotyped by long PCR-ribotyping, random amplified polymorphic DNA (RAPD) analysis, and genomic DNA restriction fragment length polymorphism (RFLP) analysis by pulsed-field gel electrophoresis (PFGE). The phenotypic study characterized 22 of the strains (73%) as biotype I. A genotypic study using long PCR-ribotyping with HaeIII restriction digestion showed no polymorphisms among these 30 Hib strains, but RAPD analysis with two sets of primers demonstrated two distinctive subtypes : one typical of the strains of biotype group II and the second characteristic of the strains of biotype groups I and IV. Each RAPD group was subtyped into several genotypic groups by PFGE-RFLP with SmaI digestion. The genotyping of clinically isolated Hib strains may help to elucidate transmission routes in community infections, endemicity, and the reasons for vaccine failure.
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