1999 Fiscal Year Final Research Report Summary
Roles of neurotrophic factors derived from astrocyte in neuronal development
| Project/Area Number |
10670747
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Nagoya City University |
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Principal Investigator |
ASAI Kiyofumi Department of Bioregulation Research, Nagoya City University Medical School, Associate Professor, 医学部, 助教授 (70212462)
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| Co-Investigator(Kenkyū-buntansha) |
KATO Taiji Department of Bioregulation Research, Nagoya City University Medical School, Professor and Chairman, 医学部, 教授 (60094364)
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| Project Period (FY) |
1998 – 1999
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| Keywords | Glia Maturation Factor / Gene / Cloning |
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| Research Abstract |
(1) Cloning of human GMF-gamma cDNA
We isolated a human GMF-gamma cDNA from human fetal brain cDNA library. One of the clones contained a 573 nucleotide long insert and an open reading frame of 426 bp encoding a deduced protein of 142 amino acids.
(2) Expression of human GMF-beta and GMF-gamma
The cDNA of human GMF-beta and GMF-gamma were inserted to pAED4 expression vector and introduced to E. coli BL21(DE3). The expressed proteins were purified and subjected to antibody production.
(3) Production of anti-human GMF-beta and GMF-gamma antibodies
Recombinant human GMF-beta and GMF-gamma were used as immunogens for the production of polyclonal antibodies. The each anti-serum recognized human GMF-beta and GMF-gamma respectively, but either of them did not react to rat GMF-beta or GMF-gamma.
(4) Cloning of rat GMF-gamma cDNA
We isolated a rat GMF-gamma cDNA from rat pulmonary artery cDNA library. One of the clones contained a 553 nucleotide long insert and an open reading frame of 426 bp encoding a deduced protein of 142 amino acids.
(5) Expression of rat GMF-beta and GMF-gamma
The cDNA of rat GMF-beta and GMF-gamma were inserted to pAED4 expression vector and introduced to E. coli BL21(DE3). The expressed proteins were purified and subjected to antibody production.
(6) Production of anti-rat GMF-beta and GMF-gamma antibodies
Anti-rat GMF-beta and GMF-gamma serum were produced by the same way of human GMF-beta and GMF-gamma. The anti-sera recognized rat GMF-beta and GMF-gamma respectively.
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[Publications] Katano, H., Fujita, K., Kato, T., Asai, K., Kawamura, Y., Masago A., and Yamada, K.: "Traumatic injury in vitro induces IEG mRNAs in cultured glial cells, suppressed by co-culture with neurons."Neuro Report. 10. 2439-2448 (1999)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Sobue, K., Yamamoto, N., Yoneda, K., Fujita, K., Miura, Y., Asai, K., Tsuda, T., Katsuya, H., Kato, T.: "Molecular cloning of two bovine aquaporin-4 cDNA isoforms and their expression in brain endothelial cells."Biochimica et Biophysica Acta 1489. 393-398 (1999)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Sobue, K., Yamamoto, N., Yoneda, K., Hodgson, M. E., Yamashiro, K., Tsuruoka, N., Tsuda, T., Katsuya, H., Miura, Y., Asai, K., Kato, T: "Induction of Blood-brain barrier properties in immortalized bovine brain endothelial cells by astrocytic factors."Neurosci. Res.. 35. 155-164 (1999)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Fuse, T., Tanikawa, M., Nakanishi, M., Ikeda, K., Tada, T., Inagaki, H., Asai, K., Kato, T., Yamada, K.: "p27kip1 expression by contact inhibition as a prognostic index of human glioma."J. Neurochem.. (in press).
Description
「研究成果報告書概要(欧文)」より
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[Publications] Tsuiki, H., Asai, K., Yamamoto, M., Fujita, K., Inoue, Y., Kawai, Y., Tada, T., Moriyama, A., Wada, Y., Kato, T.: "Cloning of a rat glia maturation factor-γ(rGMFG)cDNA and its expression of the mRNA and protein in rat organs."J. Biochem.. (in press).
Description
「研究成果報告書概要(欧文)」より
