1999 Fiscal Year Final Research Report Summary
Analysis of cutaneous immune cells in IL-7, IL-8 transgenic mice
| Project/Area Number |
10670780
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Dermatology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
NAKAMURA Kouichiro The University of Tokyo, Faculty of Medicine Lecturer, 医学部・附属病院, 講師 (60175502)
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| Project Period (FY) |
1998 – 1999
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| Keywords | IL-7 / IL-8 / transgenic mouse / Langerhans cell |
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| Research Abstract |
The function of Langerhhans cells (LC) and dendritic cells was examined using IL-7, MCP-1 transgenic (Tg) mice or wild type mice The author clarified (1) when epidermal LC were collected from these mice and TNF-α secreted by cultured LC was detected after stimulation of lipopolysaccharide or SEB, TNF-α was significantly upregulated in LC derived from MCP-1 Tg mice compared with wild type mice, (2) that , in IL-7 Tg mice, there were significant increased numbers of densritic T cells which expressed αβ or γδ TCR, (3) that, in wild type mice, Thy-1positive epidermal T cells produced IFN-γ after stimulation of IL-12 and IL-18, and (4) that cultured LC expressed CD40 ligand (CD40L) in wild type mice. These data revealed the importance of cytokines in the maturation and differentiation of cutnaous dendritic cells in the local inflammation of the skin.
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Research Products
(13 results)
