• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2000 Fiscal Year Final Research Report Summary

Cell kinetic study of epidermal keratinocyte

Research Project

Project/Area Number 10670783
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Dermatology
Research InstitutionTOYAMA MEDICAL AND PHARMACEUTICAL UNIVERSITY

Principal Investigator

MOROHASHI Masaaki  TOYAMA MEDICAL AND PHARMACEUTICAL UNIVERSITY Faculty of Medicine Professor, 医学部, 教授 (50018719)

Co-Investigator(Kenkyū-buntansha) TOYODA Masahiko  TOYAMA MEDICAL AND PHARMACEUTICAL UNIVERSITY Hospital Research Associate, 附属病院, 助手 (00251885)
OHTSUYAMA Minoru  TOYAMA MEDICAL AND PHARMACEUTICAL UNIVERSITY Hospital Research Associate, 附属病院, 助手 (10213787)
MATSUI Chihiro  TOYAMA MEDICAL AND PHARMACEUTICAL UNIVERSITY Hospital Research Associate, 附属病院, 講師 (10181679)
Project Period (FY) 1998 – 2000
KeywordsEpidermal Sheet / Brdu / Stem Cell / 紫外線
Research Abstract

This project is divided in following three parts to observe 1) the kinetic of keratinocyte two-dimensionally in epidermal sheet. 2) the marker of the stem cell and transit amplifying cell in epidermal sheet. 3) the changes in the kinetic of keratinocyte that was affected DNA damage by UVB irradiation or carcinogen. At the start, we tried to minimize the degeneration of basement membrane zone protein during making epidermal sheet. Best condition for hairless mouse skin is soaking in 1MNaBr at 37℃ for 1 hour. The expression of type 19 keratin and b1 integrin, both were reported as good marker for stem cell, was tested with epidermal sheet. Though the expression of both was detected in part of hair follicules, that was hardly detected in interfollicular epidermis. Stem cell also has been thought as rarely dividing cell. During wound repair, Stem cells should synthesize DNA and make mitosis. Since we did not find the suitable marker for distinction of stem cell, we adopted to count DNA synthesizing cell number by Brdu labeling which should include stem cell. Most of Brdu positive cells were found at the edge of surgical wound with no specific localization or relation to skin appendages. Next, we tried to discriminate between terminal differentiating keratinocyte and stem cell under the up-regulating condition of DNA synthesis, UVB irradiation or carcinogenic promotion. Both stimuli caused uniform Brdu labeling throughout basal keratinocyte in epidermal sheet and the difference of labeling intensity between differentiating keratinocyte and stem cell.
Epidermal sheet method should be a powerful tool for the two-dimensional evaluation of keratinocyte kinetics with milder condition for epidermal splitting and confocal microscopy.

  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] Onuma H,Matsui C,Morokashi M.: "Enhanced expression of SCF in the dermis is a prognostic factor for the regression of urticaria pigmentosa."Eur J Dermatol. 9・8. 629-32 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kagoura M,Matsui C,Morohashi M.: "Phytol is a novel tumor promoter on ICR mouse skin."Jpn J Cancer Res.. 90・4. 377-84 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 小沼博義,松井千尋,諸橋正昭: "表皮創傷治癒過程における細胞動態の変化-epidermal sheetを用いたBrdU染色による定量的解析-"日本皮膚科学会雑誌. 109・7. 1015-19 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Onuma H, Matsui C, Morokashi M.: "Cell kinetic change during epidermal wound healing : Quantitative analysis using Brdu in the epidermal sheet."Jpn J Dermatol. 109(7). 1015-1019 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kagoura M, Matsui C, Morohashi M.: "Phytol is a novel tumor promoter on ICR mouse skin."Jpn J Cancer Res.. Apr ; 90(4). 377-384 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Onuma H, Matsui C, Morokashi M.: "Enhanced expression of SCF in the dermis is a prognostic factor for the regression of urticaria pigmentosa."Eur J Dermatol. Dec ; 9(8). 629-632 (1999)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 2002-03-26  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi