1999 Fiscal Year Final Research Report Summary
Antigen Analysis of Latex Allergy
| Project/Area Number |
10670813
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Dermatology
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| Research Institution | Fujita Health University |
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Principal Investigator |
MATSUNAGA Kayoko Fujita Health University, School of Medicine, Assistant Pfofessor, 医学部, 講師 (80131192)
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| Project Period (FY) |
1998 – 1999
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| Keywords | Latex allergy / Contact urticaria / Skin prick test / Antigen analysis / ILZSA / Immunoblot / Specific lgE antibody / Inhibition immunoblot |
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| Research Abstract |
1) We performed alaSTAT AlaBLOT Specific lg E Assay using sera of 33 cases with latex allergy. We found several bands between 14.7kD and 113kD. The extract of a commercial latex glove inhibited most of these bands.
2) Immunoblot assay against the commercial latex glove using the same sera showed several bands. The extract inhibited one band below 7.2kD, and 28, 37 and 49 kDs bands. It inhibited 23kD band of non-ammoniated latex immunoblot using the same sera.
3) The extract of the commercial latex glove and non-ammoniated latex had common bands, one below 7.2kD, and three bands at 15, 30, and 51kD.
These results showed that our cases had two or three major latex allergens. They are Hev b 6.03 or Hev 8 of 14.7kD, and a band of 30kD which is related to latex fruit syndrome. Two band against the latex glove extract were inhibited by the latex glove extract itself. One was below 7.2kD and the other was 37kD. We suppose that they are Hev b 6.02(hevein, 47.kD) and Hev b 3(βー13-glucanese, 37kD). Inhibition immunoassay against latex glove or related fruits using havein, the related fruits or vegetables will show the mechanism of cross-reactivity between latex and related plants.
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