2000 Fiscal Year Final Research Report Summary

The investigation on the relation between the changes of tumor glucose metabolism and the apoptosis after irradiation-evaluated by measuring the lactic acid using ^1H-MRS-

Research Project

Project/Area Number	10670839
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Radiation science
Research Institution	Fukui Medical University
Principal Investigator	YOSHIDA Masanori Fukui Medical University, Medical Hospital, Lecturer, 医学部・附属病院, 講師 (00166969)
Co-Investigator(Kenkyū-buntansha)	KIMURA Hirohiko Fukui Medical University, Faculty of Medicine, Lecturer, 医学部, 講師 (10242596)
Project Period (FY)	1998 – 2000
Keywords	^1H-MRS / IN VIVO / LACTATE / APOPTOSIS / RADIOTHERAP
Research Abstract	Glucose metabolism of malignant tumor is generally increased, and anaerobic glucose metabolism is superior to the aerobic metabolism. Lactate is a main metabolite by the anaerobic metabolism. By measuring thelactate, they should be able to evaluate the activity of the tumor. We carried out this research in order to clarify the relationship between the changes of tumor glucose metabolism and the apoptosis after irradiation. The lactate concentration of the radiation induced fibrosarcoma (RIF-1) transplanted to the mouse was measurable by using ^1H-MRS.The lipid content of the intratumorale was abounding for the lymphoma, the lactate concentration of the human lymphoma transplanted to the nude mouse was not able to be measured by ^1H-MRS. The ratio of the lactale concentration of the RIF-1 tumor before and after glucose injection was calculated and was used as a standard of the glucose metabolism of the tumor. There was no change of the lactate ratio by after 6 hours of the irradiation (20Gy). The lactate ratio is lowering after 12 hours of the irradiation. In the histological evaluation by a H-E stain, there was no change by after 24 hours of the irradiation. From this result, it seemed to be possible to predict the radiation effect from lactate evaluation by ^1H-MRS. In the RIF-1 tumor, the appearance of the apoptosis was not confirmed by TUNEL method.