2000 Fiscal Year Final Research Report Summary
Does a novel hypoxic cell radiosensitizer RK28 cysteine conjugate have tumor affinity in vivo?
| Project/Area Number |
10670853
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Radiation science
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| Research Institution | Yamaguchi University |
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Principal Investigator |
EBE Kazuyu Yamaguchi University Hospital, Assistant Professor, 医学部・附属病院, 講師 (90213579)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUNAGA Naofumi Yamaguchi University School of Medicine, Professor, 医学部, 教授 (40157334)
KARINO Yuichi Yamaguchi University Hospital, Research Associate, 医学部・附属病院, 助手 (30186035)
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| Project Period (FY) |
1998 – 2000
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| Keywords | radiation / hypoxic cell / radiosensitizer / chromatography |
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| Research Abstract |
We have reported that RK28 cysteine conjugate (metabolite e) might be a novel hypoxic cell radiosensitizer as its concentration in tumor was higher than in serum on the rabbit VX2 tumor system. In this study, we report the results of its isolation and collection from rabbit urine after RK28 intravenous injection and pharmacokinetic studies for RK28 cysteine conjugate on the nude mouse KM1 human oral cancer system.
(1) RK28 and major metabolites were analyzed using high performance liquid chromatography (HPLC) with tetrabutylammonium phosphate solution (TBAP). RK28 cysteine conjugate was isolated using a LH20 column, a G10 column, and an ODS column without TBAP.A total amount of 211 μg was collected.
(2) The nude mouse KM1 human oral cancer system was newly developed for pharmacokinetic studies for RK28 cysteine conjugate
(3) Concentrations of RK28 cysteine conjugate in KM1 tumor, in serum, in urine of 2 nude mice after intravenous injection were analyzed, but it was not detected. It seems that administration doses were too little
(4) Collection methods for RK28 cysteine conjugate and a newly developed nude mouse KM1 tumor system were publicated.
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