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2000 Fiscal Year Final Research Report Summary

Molecular mechanisms of circadian rhythm system and sleep disorders

Research Project

Project/Area Number 10670914
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Psychiatric science
Research InstitutionSaitama Medical School

Principal Investigator

IKEDA Masaaki  Saitama Medical School, Assistant Prof., 医学部, 講師 (80232198)

Co-Investigator(Kenkyū-buntansha) HONMA Sato  Hokkaido Univ.Medical School, Associate Prof, 医学部, 助教授 (20142713)
ORIMO Akira  Saitama Medical School, Assistant Prof., 医学部, 講師 (70275866)
YOSHIMURA Kazunori  Saitama Medical School, Assistant Prof., 医学部, 助手 (20158497)
Project Period (FY) 1998 – 2000
Keywordscircadian rhythm / BMAL1 / clock gene / suprachiasmatic nuclei / sleep disorder / BMAL2 / gene analysis / transcription factor
Research Abstract

1) We have cloned a BAC clone for human BMAL1 gene and determined its sequence. It revealed that human BMAL1 coding region consists from 17 exons and several BMAL1 variants are products from splicing variants.
2) We have cloned a BAC clone for mouse BMAL1. Its coding region splits over 30kb.
3) Mouse BMAL1 promoter region was sequenced. We identified the promoter/enhancer region of BMAL1.
4) The transcription start sites for BMAL1 have been identified.
5) Polymorphism of BMAL1 gene from sleep disorders were searched. No polymorphism were identified in the BMAL1 coding region.
6) Two-hybrid system to test the interaction of clock gene products have been established.
7) Luciferase assay system to test the ability to activate mPer1 promoter have been established.
8) Fusion proteins of clock gene have been prepared for examining the interaction of clock gene products.
9) A nobel bHLH-PAS factor have been cloned and designated BMAL2.
10) The expression, chromosomal localization and cellular localization of BMAL2 were examined.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Ikeda,M,Yu,W et al: "cDNA Cloning of a Novel bHLH-PAS Transcription Factor Superfamily Gene, BMAL2 ; Its mRNA Expression, Subcellular Distribution, and Chromosomal Localizatio. "Biochem Biophys Res Commun. 275. 493-502 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Masubuchi S,Honma S et al: "Clock genes outside the suprachiasmatic nucleus involved in manifestation of locomotor activity rhythm in rats "Eur J of Neurosci. 12. 4206-4214 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Abe H,Honma S et al: "Clock gene expressions in the suprachiasmatic mucleus and other areas of the brain during thythm splitting in CS mice"Mol.Brain Res. 87(1). 92-99 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 池田正明: "体内時計は転写因子で刻まれる?"バイオサイエンスとインダストリー. 58. 16-21 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 池田正明: "-生物時計関連遺伝子-PASドメインとPAS因子-PAS因子は環境適応素子として生まれた-"日本時間生物学会会誌. 6. 12-25 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 池田正明: "BMAL1と生体リズム"日本神経精神薬理学雑誌(印刷中). 20. 189-198 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ikeda, M, Yu, W, Hirai, M, Ebisawa, T, Honma, S, Yoshimura, K, Honma K, and Nomura M: "cDNA Cloning of a Novel bHLH-PAS Transcription Factor Superfamily Gene, BMAL2 ; Its mRNA Expression, Subcellular Distribution, and Chromosomal Localization"Biochem Biophys Res Commun. 275. 493-502 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Masubuchi S, Honma S, Abe H, Ishizaki K, Namihira M, Ikeda M, and Honma K: "Clock genes outside the suprachiasmatic nucleus involved in manifestation of locomotor activity rhythm in rats"Eur J of Neurosci. 12. 4206-4214 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Abe H, Honma S, Namihira M, Masubuchi S, Ikeda M, Ebihara S, and Honma K: "Clock gene expressions in the suprachiasmatic mucleus and other areas of the brain during thythm splitting in CS mice"Mol.Brain Res.. 87. 92-99 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Masaaki Ikeda: "Circadian timekeeping is generated by transcription factors"Bioscience and Bioindustry. 58 (in Japanese). 242-247 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Masaaki Ikeda: "PAS domain and PAS factors- an aspecto of PAS factors as modules for adaptation to embironment"Japanese Society for Chronobiology. 6 (in Japanese). 12-25 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Masaaki Ikeda: "BMAL1 and circadian rhythm"Jpn J Neuropsychopharmacol. 20 (in Japanese). 189-198 (2000)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2002-03-26  

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