2000 Fiscal Year Annual Research Report

T細胞抗原受容体CDR3解析による造血細胞移植後免疫再構築のモニタリング

Research Project

Project/Area Number	10670932
Research Institution	AKITA UNIVERSITY SCHOOL OF MEDICINE
Principal Investigator	廣川誠秋田大学, 医学部, 助手 (50241667)
Co-Investigator(Kenkyū-buntansha)	川端良成秋田大学, 医学部, 医員(臨床)
Keywords	T細胞抗原受容体 / レパートリー / CDR3 / peripheral expansion / T細胞再構築 / 免疫不全症 / クローン / 造血幹細胞移植
Research Abstract	ヒト同種造血幹細胞移植後におけるT細胞再生の機序について、T細胞受容体(TCR)レパートリーの観点から解析した。移植後2ないし3ヶ月めにT細胞抗原受容体α鎖可変領域(T-cell receptor α-chain variable region,TCRAV)およびT細胞抗原受容体β鎖可変領域(T-cell receptor β-chain variable region,TCRBV)レパートリーのskewingが観察され、その程度は成人においてより顕著であった。TCRAVおよびTCRBVレパートリーのゆがみはクローナルなT細胞の増加に起因し、TCR-β鎖complementarity-determining region 3(CDR3)領域にコンセンサスシークエンスがみられる症例が存在した。CDR3スペクトラタイピングによりレシピエントの免疫能はαβ型T細胞レパートリーの回復に依存していることが示された。これらの結果は移植後早期におけるT細胞再生経路の主体は動物実験モデルでその存在が証明されているperipheral expansionであることを示唆している。

Research Products
(6 results)

All Other

All Publications (6 results)

[Publications] Hirokawa M: "Extensive clonal expansion of T lymphocytes causes contracted diversity of the complementarity-determining region 3 and a skewing of T-cell receptor repertoires after allogeneic hematopoietic cell transplantation"Bone Marrow Transplantation . (in press).
[Publications] Horiuchi T: "Identification of the T-cell clones expanding within both CD8+CD28+ and CD8+CD28- T-cell subsets in recipients of allogeneic hematopoietic cell grafts and its implication in post-transplant skewing of TCR repertoire"Bone Marrow Transplantation. (in press).
[Publications] Hirokawa M: "Delayed recovery of CDR3 complexity of the T-cell receptor-β chain in recipients of allogeneic marrow transplants who had virus-associated interstitial pneumonia : monitor of T-cell function by CDR3 spectratyping"Journal of Allergy and Clinical Immunology. 106. 32-39 (2000)
[Publications] Matsutani T: "Restricted usage of T-cell receptor α-chain variable region (TCRAV) and T-cell receptor β-chain variable region (TCRBV) repertoires after human allogeneic hematopoietic transplantation"British Journal of Haematology. 109. 759-769 (2000)
[Publications] Hirokawa M: "Reconstitution of γδ T-cell repertoire diversity after human allogeneic hematopoietic cell transplantation and the role for peripheral expansion of mature T-cell population in the graft"Bone Marrow Transplantation. 26. 177-185 (2000)
[Publications] 廣川誠: "ヒト同種造血幹細胞移植におけるT細胞レパートリーの再構築"血液・腫瘍科. (印刷中).

2000 Fiscal Year Annual Research Report

T細胞抗原受容体CDR3解析による造血細胞移植後免疫再構築のモニタリング

Principal Investigator

廣川 誠 秋田大学, 医学部, 助手 (50241667)

Research Products

[Publications] Hirokawa M: "Extensive clonal expansion of T lymphocytes causes contracted diversity of the complementarity-determining region 3 and a skewing of T-cell receptor repertoires after allogeneic hematopoietic cell transplantation"Bone Marrow Transplantation . (in press).

[Publications] Horiuchi T: "Identification of the T-cell clones expanding within both CD8+CD28+ and CD8+CD28- T-cell subsets in recipients of allogeneic hematopoietic cell grafts and its implication in post-transplant skewing of TCR repertoire"Bone Marrow Transplantation. (in press).

[Publications] Matsutani T: "Restricted usage of T-cell receptor α-chain variable region (TCRAV) and T-cell receptor β-chain variable region (TCRBV) repertoires after human allogeneic hematopoietic transplantation"British Journal of Haematology. 109. 759-769 (2000)

[Publications] Hirokawa M: "Reconstitution of γδ T-cell repertoire diversity after human allogeneic hematopoietic cell transplantation and the role for peripheral expansion of mature T-cell population in the graft"Bone Marrow Transplantation. 26. 177-185 (2000)

[Publications] 廣川誠: "ヒト同種造血幹細胞移植におけるT細胞レパートリーの再構築"血液・腫瘍科. (印刷中).

廣川誠秋田大学, 医学部, 助手 (50241667)