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1999 Fiscal Year Final Research Report Summary

The study on molecular biological mechanisms and therapy of sodiam and acid disturbance in renal failure

Research Project

Project/Area Number 10671000
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Kidney internal medicine
Research InstitutionKumamoto University

Principal Investigator

NONOGUCHI Hiroshi  University Hospital, Kumamoto University, Associate Professor, 医学部・附属病院・第三内科, 講師 (30218341)

Co-Investigator(Kenkyū-buntansha) 野々口 博史  熊本大学, 医学部・附属病院・第三内科, 講師 (30218341)
Project Period (FY) 1998 – 1999
KeywordsChronic renal failure / Vesopressin / V2 receptor / Via receptor / NKCC1 / Collecting cluct / Osmolality
Research Abstract

Patients with chronic renal failure showed decreased urinary excretion of sodium and acid. Several ion transporters in the kidney participate in sodium and acid excretion. We focused on secretory type Na-K-2Cl cotransporter (NKCC1). To know the role of NKCC1 in the regulation of body fluid homeostasis, we investigated the distribution of NKCC1 along the nephron in mouse and rat. NKCC1 mRNA expression was most abundant in inner medullary collecting ducts (IMCD) in mouse and outer medullary collecting ducts (OMCD) in rat. Chronic metabolic acidosis induced by the administration of NH4C1 and two-days dehydration caused a significant increases of NKCC1 mRNA expression in collecting ducts and NKCC1 protein expression in OMCD. Next, to examine the mechanisms of the up-regulation of NKCC1 mRNA expression by dehydration, the effects of hyperosmolality and vasopressin (AVP) on its expression were studied. Hyperosmolality and AVP increased NKCC1 mRNA expression in OMCD. These results show that AVP, directly or indirectly through the increase in medullary osmolality, regulates NKCC1 expression. Since V2 vasopressin receptors were downregulated in chronic renal failure, AVP plays an important role in sodium and acid excretion in patients with renal failure.

  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] H. Nonoguchi: "Regulation of the renal Na/K/2cl cotransporter gene physiological modulation in health and abnormal function in disease"Exp. Nephrol. 6. 272-276 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H. Nonoguchi: "Renal effects of temocapril hydrochloride (CS-622) in patients with benign nephrosclerosis"Nephrology. 4. 183-186 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M. Takayama: "Acute and chromic effects of hyperosomalality on mRNA and protein expressions and the activity of Na-K-ATPase in the IHCD"Exp Nephrol. 7. 295-303 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K. Itoh: "Gene regulation of atrial natriuretic peptide A, B, and C receptors in rat glomerule"Exp. Nephrol.. 7. 328-336 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y. Nakayama: "Intranephron distributiion and regulation of endothelin-converting enzyme-1 in cyclosporin A-induced acute renal failure in rats"J. Am. Soc. Nephrol.. 10. 562-571 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y. Nakayama, H. Nonoguchi, S. Kiyama, M. Ikebe, Y. Tashima, K.Shimada, K. Tanzawa, K, Tomita: "Intranephron distribution and regulation of endothelin-converting enzyme-1 in cyclosporin A-induced acute renal failure in rats"J. Am. Soc. Nephrol. 10. 562-571 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K. Itoh, H. Nonoguchi, N. Shiraishi, K. Tomita: "Gene regulation of atrial natriuretic peptide A, B, and C receptors in rat glomeruli"Exp. Nephrol. 7. 328-336 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M. Takayama, H. Nonoguchi, T. Yang, K. Machida, Y. Terada, A. Owada, K. Tomita, F. Marumo: "Acute and chronic effects of hyperosmolality on mRNA and protein expression and the activity of Na-K-ATPase in the IMCD"Exp. Nephrol. 7. 295-305 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H. Nonoguchi, A. Owada, I. Umehara, S. Kiyama, Y. Terada, F. Marumo, K. Tomita: "Renal effects of temocapril hydrocholoride (CS-622) in patients with benign nephrosclerosis"Nephrology. 4. 183-186 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Nonoguchi, K. Itoh, M. Ikebe, K. Tomita: "Regulation of the renal Na/K/2C1 cotransporter gene: physiological modulation in health and abnormal function in disease"Exp. Nephrol. 6. 272-276 (1998)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2001-10-23  

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