2000 Fiscal Year Final Research Report Summary
Research on the structure and functions of human FTZ-F1β gene
| Project/Area Number |
10671039
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Endocrinology
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| Research Institution | KYUSHU UNIVERSITY |
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Principal Investigator |
YANASE Toshihiko Kyushu University, Graduate School of Medical Sciences, Associate Professor, 大学院・医学研究院, 助教授 (30239818)
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| Co-Investigator(Kenkyū-buntansha) |
NAWATA Hajime Kyushu University, Graduate School of Medical Sciences, Associate Professor, 大学院・医学研究院, 教授 (10038820)
TAKAYANAGI Ryoichi Kyushu University, Graduate School of Medical Sciences, Associate Professor, 大学院・医学研究院, 教授 (30154917)
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| Project Period (FY) |
1998 – 2000
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| Keywords | FTZ-F1β / Ad4BP |
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| Research Abstract |
FTZ-F1β had been implicated to be a counterpart of Ad4BP and mainly expressed in liver and pancreas. We elucidated the structure of the human FTZ-F1β gene. We determined the exonic sequences including the exon/intron baundaries and the sequences at the 5'-flanking region. The gene is at least 50 Kb long and is split into 8 exons including a non-coding exon 1. The overall structural organization of human FTZ-F1β gene was very similar to that of human Ad4BP/SF-1 gene, suggesting that both genes are derived from common ancestor gene. In addition, a preliminary study of transcriptional regulation of human FTZ-F1β gene in Hep G2 cells using a series of deletion analysies of the 5'-flanking region of human FTZ-F1β gene revealed a upstream region of -1743 to -1040 bp from the transcriptional statsite to be essential for the basal transcription of the human FTZ-F1β gene in Hep G2 cells.
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