1999 Fiscal Year Final Research Report Summary
Anti-angiogenic drug AGM1470 suppresses smooth muscle cell migration induced by endothelial PDGF.
| Project/Area Number |
10671102
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | THE UNIV. OF TOKYO |
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Principal Investigator |
HOSOI Yutaka The University of Tokyo, Faculty of Medicine, Assistant., 医学部・附属病院, 助手 (40311625)
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| Co-Investigator(Kenkyū-buntansha) |
HATAKEYAMA T. The University of Tokyo, Faculty of Medicine, Assistant, 医学部・附属病院, 助手 (60291324)
MIYATA T. The University of Tokyo, Faculty of Medicine, Assistant Professor, 医学部・附属病院, 講師 (70190791)
SHIGEMATSU Hiroshi The University of Tokyo, Faculty of Medicine, Associate Professor, 医学部・附属病院, 助教授 (40134556)
重松 邦広 東京大学, 医学部・附属病院, 助手
SHIGEMATSU K. The University of Tokyo, Faculty of Medicine, Assistant
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| Project Period (FY) |
1998 – 1999
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| Keywords | Neovascularization / Hyperplasia of anast / Endothelium, Vascular / Smooth muscle cell / Shear stress |
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| Research Abstract |
OBJECTIVES: To examine the effects of the anti-angiogenic drug AGM1470 on smooth muscle cell (SMC) migration activity stimulated by endothelial cell (EC-derived mitogen. MATERIALS AND METHODS: Study 1 ; EC's were cultured under pulsatile flow using MCDB151 medium. From the supernatant of these EC dishes we devised two types of conditioned medium; anti-PDGF(+) containing 10 micrograms/ml anti-PDGF antibody, and anti-PDGF(-) containing no antibody. SMC's were cultured using both media. Study 2 ; EC's were cultured under the same conditions using both types of medium; MCDB151 medium containing 10 ng/ml AGM1470, and MCDB151 medium alone. After the AGM1470 concentration had been adjusted to 10 ng/ml, SMC's were cultured using each medium ; AGM-exposed EC and AGM-non-exposed EC. SMC colony spreading distances were measured as an index of mitogenic activity for 4 days. RESULTS: Study 1 ; the anti-PDGF(-) group showed an apparently greater spreading distance than the anti-PDGF(+) group. Study 2 ; the AGM-non-exposed EC group showed a significantly greater spreading distance than the AGM-exposed EC group. However, MTT assay revealed no differences in proliferation between the two groups. CONCLUSION: AGM1470 suppresses the EC production of this PDGF-like mitogen as well as SMC migration activity.
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