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1999 Fiscal Year Final Research Report Summary

Experimental study of traumatic DIC -NO production in tissue and histopathology following hemorragic invasion in the rat-

Research Project

Project/Area Number 10671108
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General surgery
Research InstitutionFukui Medical University

Principal Investigator

SUGA Hiroyasu  Fukui Medical University, Emergency Medicine, Assistant, 医学部・附属病院・救急部, 助手 (10266752)

Co-Investigator(Kenkyū-buntansha) TAKAO Nakagawa  Fukui Medical University, Emergency Medicine, Assistant Professor, 医学部・附属病院・救急部, 助教授 (40075578)
Project Period (FY) 1998 – 1999
Keywordshemorrhagic invasion / endotoxin adminstration / NO production / NO synthase inhibitors / histopathology / coagulation-fibrinolytic system / cytokine / traumatic DIC
Research Abstract

Previously, in order to study the pathogenesis of traumatic DIC, we investigated and confirmed the onset of DIC following hemorrhagic invasion in the rat, and at that time we noticed a rapid increase in nitric oxide (NO) in the tissue in the early stage after the invasion. It was suggested that the increase in NO production would serve as an indication for diagnosis of traumatic DIC. In this study, we investigated the time course and mechanism of NO production in the tissue and its histopathology following hemorragic invasion in the rat, in comparsion with the rat following endotoxin administration. Then we investigated therapeutic effects of NO synthase (NOS) inhibitors for these morbid states.
In the fiscal year 1998, the time course of NO production in the tissue following hemorrhagic invasion (bleeding of 30% of predetermined total blood volume) in the rat was recorded with an NO-selective electrode(φ200μm, Intermedical Co.) set in the kidney tissue, comparing with that rat followin … More g endotoxin administration (LPS, E, coli, 10mg/kg iv). In the hemorrhagic invasion, increase in NO production in the early stage was observed and it was suggested strongly to be due to constitutive NOS (cNOS) by the investigation with NOS inhibitors. On the contrary, in the endotoxin administration NO production was not observed at all in the early stage, but it begun to increase gradually 2〜3hr after the administration and reached a peak after 4〜6hr, and it was suggested strongly to be due to inducible NOS (iNOS). We could detect a characteristic ESR signal of NO in the sample solution of the kidney tissue following both the invasions.
In the fiscal year 1999, therapeutic effects of NOS inhibitors and other drugs for these morbid states were investigated. NOS inhibitors have been tried to treat for various types of circulatory shock. In this experiment NOS inhibitors reduced partly the changes in the coagulation-fibrinolytic system and plasma cytokine levels induced by the invasions, but did not ameliorate significantly histopathological changes. On the other hand, antithrombin III ameliorated changes in coagulation-fibrinolytic system and plasma cytokine levels, and also histopathological changes, too. Theraputic effects of NOS inhibitors for these morbid states should be further investigated in detail on types, dosages, time of treatment of NOS inhibitors, considering two sided roles of NO effect. Less

  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] Suga,H.et al: "Effects of No synthase inhibitors and antithrombin III on oytokine levels in blood and histopathological findings following hemorrhagic invasion in comparison with septic invasion"Proceedings of the 5th World Congress of Trauma, shock, Inflammation and Sepsis. 745-750 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Suga,H.et al: "Comparison of nifrie oxide (NO) production in the tissue following hemorrhagic invasion and endotoxin administration in rats in relation to the pathogenesis of traumatic DIC"J Tokyo Wom Med Univ. 70 (3) (in -press). (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Suga,H.et al: "Effect of nitric oxide synthase inhibitors on various types of invasion in the rat: on coagulofibrinolytic system, cytokine levels and histopathological findings"The Biology of Nitric Oxide. Part 7 (in -press). (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakagawa T. Suga,h.et al: "Experimental study on the onset of traumatic DIC : changes in the coagulofibrinolytic system, blood cytokine. levels and histopathological findings following hemorrhage in rats"J Tokyo Wom Med Univ. 69. 396-403 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 須賀弘泰ほか: "敗血症性DICラットに対するNOS阻害剤の治療効果"日本ショック学会雑誌. 14(2). 19-23 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 須賀弘泰ほか: "重症膵炎とDIC"バイオメディカル. 9. 39-50 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 須賀弘泰ほか: "外傷性DIC発症機序の実験的検討―侵襲時の組織NO産生と病理組織学的変化の関連について"バイオメディカル. 8. 41-48 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 須賀弘泰ほか: "出血性ショックを伴ったDICの3剖検例―早期治療例1例無治療例2例"バイオメディカル. 7. 9-18 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Suga,H.et al: "Nitric oxide production and occurrence of disseminated intravascular coagulation in the bleeding invasion rat"The Biology of Nitric oxide. Part 6. 179 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Suga,H.et al: "Experimental study on the pathogenesis of traumatic DIC : Changes in plasma cytokine Level and coagulofibrinolytic system in the hemorrhage"Proceedings of the 4th International Congress on the Immune Consequences of trauma, shock and sepsis. 143-147 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Suga H., Nakagawa T., Miyoshi N., Deguchi Y., Imamura Y., Fukuda M.: "Effects of NO synthase inhibitors and antithorombin III on cytokine levels in blood and histopathological findings following hemorrhagic invasion in comparison with septic invasion"Proceedings of the 5th World Congress on Trauma, Shock, Inflammation and Sepsis. 745-750 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hiroyasu Suga, Takao Nakagawa, Norio Miyoshi, Yoshiaki Imamura, Masaru Fukuda: "Comparison of nitric oxide (NO) production in the tissue following hemorrhagic invasion in comparison with septic invasion"J Tokyo Wom Med Univ. 70(3)(in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H. Suga, T. Nakagawa, N. Miyoshi, Y. Deguchi, Y. Imamura, M. Fukuda: "Effect of nitric oxide synthase inhibitor on various types of invasion in the rat: on coagulofibrinolytic system, cytokine levels and histopathological findings"The Biology of Nitric Oxide. Part 7(in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takao Nakagawa, Hiroyasu Suga, Norio Miyoshi, Yoshiaki Imamura, Masaru Fukuda, Yoshizumi Deguchi, Tadashi Suzuki: "Experimental study on the onset of traumatic DIC: changes in the coagulofibrinolytic system, blood cytokine levels and histopathological findings following hemorrhage in rats"J Tokyo Wom Med Univ. 69(7,8). 396-403 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H. Suga, T. Nakagawa, N. Miyoshi, Y. Deguchi, Y. Imamura, M. Fukuda: "Therapeutic effects of NOS inhibitors on the rat with septic DIC (In Japanese)"J Jap Shock Soc. 14(2). 19-23 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H. Suga, T. Nakagawa, Y. Deguchi, S. Ota, K. Senda, N. Kubota: "Severe pancreatitis and DIC (In Japanese)"Biomedical. 9. 39-50 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H. Suga, T. Nakagawa, T. Yokoyama, Y. Deguchi, R. Muraoka, M. Fukuda, N. Miyoshi, M. Imamura: "Experimental investigation on pathogenesis of traumatic DIC -on relation ship between NO production in tissue and histopathological changes following invasions- (In Japanese)"Biomedical. 8. 41-48 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H. Suga, T. Nakagawa, T. Yokoyama, Y. Deguchi, R. Muraoka, M. Fukuda, N. Miyoshi, M. Imamura, S. Noriki, K. Nakakuki, H. Naiki: "3 autopsied cases following hemorrhagic shock -one early treated case and 2 non-treated cases- (In Japanese)"Biomedical. 7. 9-18 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Suga, H., Nakagawa, T., Yokoyama, T., Deguchi, Y., Miyoshi, N., Imamura, Y., Fukuda, M.: "Nitric oxide production and occurrence of disseminated intravascular coagulation in the bleeding invasion rats"The Biology of Nitric Oxide. Part 6. 179 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Suga, H., Nakagawa, T., Yokoyama, T., Miyoshi, N., Imamura, Y and Fukuda, M.,: "Experimeutal study on the pathogenesis of traumatic DIC: Changes in plasma cytokine levels and coagulofibrinolytic system in the hemorrhage"Proceedings of the 4th International Congress on the Immune Consequences of Trauma, Shock and Sepsis. 143-147 (1997)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2001-10-23  

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