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1999 Fiscal Year Final Research Report Summary

The analysis of the function of Angiogenesis Inhibiting Protein (AIP)

Research Project

Project/Area Number 10671147
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General surgery
Research InstitutionTazuke Kofukai Medical Research Institute

Principal Investigator

ADACHI Masashi  Tazuke Kofukai Medical Research Institute, 医学研究所・第5研究部, 主任研究員 (20291015)

Co-Investigator(Kenkyū-buntansha) MIYAKE Masayuki  Tazuke Kofukai Medical Research Institute, 医学研究所・第5研究部, 部長 (90250076)
Project Period (FY) 1998 – 1999
Keywordsangiogenesis / cancer / AIP
Research Abstract

Suppression of angiogenesis is of importance in cancer treatment. The molecular basis of human endothelial cell motility is obviously highly complex and is believed to be controlled by a number of molecular systems, including cell adhesion molecules, their receptors, cytoskeletal components, a junctional unit connecting the cytoskeletal components and membrane receptors, and various peptide growth factors. The aims of this study were to systematically investigate the possible involvement of proteins at the human endothelial cell surface in controlling cell motility and further identify the cell surface molecules involved in the angiogenesis. The present study utilized an approach based on the selection of HUVEC, which showed motility. We have addressed this study using functional monoclonal antibodies (MAbs), which inhibit HUVEC motility, as probes. The monoclonal antibody HM7-3 was established after immunization of mice with HUVEC, and was selected on the basis of its ability to inhibit HUVEC migration in a matrigel transwell penetration assay. HM7-3 inhibited angiogenesis using eggs and cornea of rats. cDNA cloning revealed that HM7-3 recognizes a new specific protein structure and we named this protein Angiogenesis Inhibiting Protein (AIP). However, the extent of suppression of angiogenesis is not directly related to the level of AIP expression. As AIP has several N-glycosylation sites, the function of AIP may be, in general, highly controlled by N-glycosylation. De-N-glycosylation of AIP results in the loss of functions. The further examination will be necessary for the analysis of the mechanism of AIP.

  • Research Products

    (43 results)

All Other

All Publications (43 results)

  • [Publications] Adachi, M., et al.: "Raduced integrin α3 expression as a factor of poor prognosis of patients with adenocarcinoma of the lung"Journal of Clinical Oncology. 16. 1060-1067 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Adachi, M., et al.: "A Novel staging protocol for non-small cell lung cancers according to MRP-1/CD9 and KAI1/CD82 gene expression"Journal of Clinical Oncology. 16. 1397-1406 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Huang, C., et al.: "Mutations in exon 7 and 8 of p53 as poor prognostic factors in patients with non-small cell lung cancer"Oncogene. 16. 2469-2477 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Huang, C., et al.: "Mutations of p53 and K-ras genes as prognostic factors for non-small cell lung cancer"International Journal of Oncology. 12. 553-563 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yagita, M., et al.: "Therapy-related leukemia and myelodysplasia following oral administration of etoposide for recurrent breast cancer"International Journal of Oncology. 13. 91-96 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sho, M., et al.: "Transmambrane 4 superfamily as a prognostic factor in pancreatic cancer"International Journal of Oncology. 79. 509-516 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Higashiyama, M., et al.: "KAI1/CD82 expression in non-small cell lung carcinoma is a novel, favorable prognostic factor"Cancer. 83. 466-474 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Huang, C., et al.: "Correlation of reduction in MRP-1/CD9 and KAI1/CD82 expression with recurrences in breast cancer patients"American Journal of Pathology. 153. 973-983 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Inufusa, H., et al.: "Correlation of prognosis of breast cancer patients and expression of Ley which acts as a confactor of tumor procoagulant"International Journal of Oncology. 13. 481-487 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 足立匡司: "肺癌治療における分子生物学的診断の意義-自験例(TM4SFの研究)を交えて"臨床外科. 53. 835-841 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 三宅正幸: "モノクローナル抗体の作製"外科. 60. 1567-1570 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Uchida, S., et al.: "Motility-related Protein(MRP-1/CD9 ) and KAI1/CD82 expression inversely correlate with lymph node metastasis in oesophageal squamous cell cancinoma"British Journal of Cancer. 79. 1168-1173 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ikeda, N., et al.: "Prognostic significance of angiogenesis in human pancreatic cancer"British Journal of Cancer. 79. 1553-1563 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 三宅正幸: "Transmambrance 4 Super Family (TM4SF) と肺癌"分子呼吸器病. 3. 12-18 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Miyake, M., et al.: "A novel molecular staging protocol for non-small cell lung cancer"Oncogene. 18. 2397-2404 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakamura, M., et al.: "Involvement of Galectin-3 expression in colorectal cancer prognossion and metastasis"International Journal of Oncology. 15. 143-148 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Huang, C., et al.: "Overexpression of Bax associated with mutations in the Loop-Sheet-Helix Motif of p53"American Journal of Pathology. 155. 955-965 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yang, S. -Z., et al.: "Adriamycin activates E-cadherin-mediated cell-cell adhesion in human breast cancer"International Journal of Oncology. 15. 1109-1115 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Huang, C., et al.: "p16 protein expression is associated with a poor prognosis in squamous cell carcinoma of the lung"British Journal of Cancer. 82. 374-380 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hirasawa, Y., et al.: "Natural autoantibody to MUC1 is a prognostic inducator for non-small cell lung cancer"American Journal of Respiratory and Critical Care Medicine. 161. 589-594 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Adachi, M., et al.: "Significance of integrin α5 gene expression as a prognostic factor in node negative non-small cell lung cancer"Clinical Cancer Research. 15. 96-101 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hamada, H., et al.: "A novel monoclonal antibody, H9, directed against the core protein of MUC1 Mucin"Oncology Report. 7. 225-232 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Konishi, T., et al.: "The K-ras gene regulated vascular endothelial growth factor gene expression in non-small cell lung cancers"International Journal of Oncology. 16. 501-511 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Adachi,M., Taki,T., Konishi,T., Huang,C., Higashiyama,M., Doi,O., Tsuji,T., and Miyake,M.: "Reduced Integrin α3 Expression as a Factor of Poor Prognosis of Patients with Adenocarcinoma of the Lung"J. Clin Oncol.. 16. 1060-1067 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Adachi,M., Taki,T., Konishi,T., Huang,C., Higashiyama,M., and Miyake,M.: "Novel Staging Protocol for Non-small Cell Lung Cancers According to MRP-1/CD9 and KAI1/CD82 Gene Expression"J. Clin Oncol.. 16. 1397-1406 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Huang,C., Taki,T., Adachi,M., Konishi,T., Higashiyama, M., and Miyake,M.: "Mutations in Exon 7 and 8 of p53 as Poor Prognostic Factors in Patients with Non-small Cell Lung Cancer"Oncogene. 16. 2469-2477 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Huang,C., Taki,T., Adachi,M., Konishi,T., Higashiyama, M., Kinoshita,M., Hamada,T., and Miyake,M.: "Mutations of p53 and K-ras Genes as Prognostic Factors for Non-small Cell Lung Cancer"Int. J. Oncol.. 12. 553-563 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yagita,M., Ieki,Y., Onishi,R., Huang,C., Adachi,M., Horiike,S., Konaka,Y., Taki,T., and Miyake,M.: "Therapy-related Leukemia and Myelodysplasia Following Oral Administration of Etoposide for Recurrent Breast Cancer"Int. J. Oncol.. 13. 91-96 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sho,M., Adachi,M., Taki,T., Hashida,H., Konishi,T., Huang,C., Ikeda,N., Nakajima,Y., Kanehiro,H., Hisanaga,M., Nakano,H., and Miyake,M.: "Transmembrane 4 Superfamily as a Prognostic Factor in Pancreatic Cancer"Int. J. Cancer. 79. 509-516 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Higashiyama,M., Kodama,K., Yokouchi,H., Takami,K., Adachi,M., Taki,T., Ishiguro,S., Nakamori,S., Yoshie,O., and Miyake,M.: "KAI1/CD82 Expression in Non-small Cell Lung Carcinoma Is a Novel, Favorable Prognostic Factor"Cancer. 83. 466-474 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Huang,C., Kohno,N., Ogawa,E., Adachi,M., Taki,T., and Miyake,M.: "Correlation of Reduction in MRP-1/CD9 and KAI1/CD82 Expression with Recurrences in Breast Cancer Patients"Am. J. Pathol.. 153. 973-983 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Inufusa,H., Nakatani,Y., Adachi,T., Wakano,T., Nakajima,A., Nakamura,M., Suzuki,M., Ando,O., Kurimoto,M., Miyake,M., Shindo,K., and Yasutomi,M.: "Correlation of Prognosis of Breast Cancer Patients and Expression of Ley Which Acts as a Cofactor of Tumor Procoagulant"Int. J. Oncol.. 13. 481-487 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Uchida,S., Shimada,Y., Watanabe,G., Li,Z-G., Hong T., Miyake,M., and Imamura,M.: "Motility-related Protein (MRP-1/CD9) and KAI1/CD82 Expression Inversely Correlate with Lymph Node Metastasis in Oesophageal Squamous Cell Carcinoma"Brit. J. Cancer. 79. 1168-1173 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ikeda,N., Adachi,M., Taki,T., Huang,C., Hashida,H., Takabayashi,A., Sho,M., Nakajima,Y., Kanehiro,H., Hisanaga,M., Nakano,H., and Miyake,M.: "Prognostic Significance of Angiogenesis in Human Pancreatic Cancer"Brit. J. Cancer. 79. 1553-1563 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Miyake,M., Adachi,M., Huang,C., Higashiyama,M., Kodama,K., and Taki,T.: "A Novel Molecular Staging Protocol for Non-small Cell Lung Cancer"Oncogene. 18. 2397-2404 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakamura,M., Inufusa,H., Adachi,T., Aga,M., Kurimoto,M., Nakatani,Y., Wakano,T., Nakajima,A., Hida,J-I., Miyake,M., Shindo,K., and Yasutomi,M.: "Involvement of Galectin-3 Expression in Colorectal Cancer Progression and Metastasis"Int. J. Oncol.. 15. 143-148 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Huang,C., Kohno,N., Inufusa,H., Kodama,K., Taki,T., and Miyake,M.: "Overexpression of Bax Associated with Mutations in the Loop-Sheet-Helix Motif of p53"Am. J. Pathol.. 155. 955-965 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yang,S-Z., Kohno,N., Kondo,K., Yokoyama,A., Hamada,H., Hiwada,K., and Miyake,M.: "Adriamycin Activates E-cadherin-mediated Cell-cell Adhesion in Human Breast Cancer Cells"Int. J. Oncol.. 15. 1109-1115 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Huang,C., Taki,T., Higashiyama,M., Kohno,N., and Miyake,M.: "p16 Protein Expression Is Associated with a Poor Prognosis in Squamous Cell Carcinoma of the Lung"Brit. J. Cancer. 82. 374-380 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hirasawa,Y., Kohno,N., Yokoyama,A., Kondo,K., Hiwada,K., and Miyake,M.: "Natural Autoantibody to MUC1 Is a Prognostic Indicator for Non-Small Cell Lung Cancer"Am. J. Respir. Crit. Care Med.. 161. 589-594 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Adachi,M., Taki,T., Higashiyama,M., Kohno,N., Inufusa,H., and Miyake,M.: "Significance of Integrin α5 Gene Expression as a Prognostic Factor in Node Negative Non-small Cell Lung Cancer"Clin. Cancer Res.. 6. 96-101 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hamada,H., Kohno,N., Yokoyama,A., Kondo,K., Yang,S-Z., Hiwada,K., Watanabe,K., and Miyake,M.: "A Novel Monoclonal Antibody, H9, Directed against the Core Protein of MUC1 Mucin"Oncol. Report.. 7. 225-232 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Konishi,T., Huang,C.-L., Adachi,M., Taki,T., Inufusa,H., Kodama,K., Kohno,N., and Miyake,M.: "The K-ras gene regulates vascular endothelial growth factor gene expression in non-small cell lung cancers"Int. J. Oncol.. 16. 501-511 (2000)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2001-10-23  

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