2000 Fiscal Year Final Research Report Summary
Cadaver Intrathoracic Cooling by Cold Air
| Project/Area Number |
10671267
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Thoracic surgery
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| Research Institution | Kagoshima University |
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Principal Investigator |
SHUN-ICHI Watanabe University Hospital, Faculty of Medicine. Kagoshima University Assistant Professor, 医学部・附属病院, 講師 (90264415)
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| Co-Investigator(Kenkyū-buntansha) |
TODA Riichiro University Hospital, Faculty of Medicine. Kagoshima University Research Associate, 医学部, 助手 (90284869)
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| Project Period (FY) |
1998 – 2000
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| Keywords | Cadaver intrathoracic cooling / Intrathoracic cooling / Lung transplantation / Non-heart-beating-donors / Cold air |
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| Research Abstract |
Background. If lungs could be retrieved for transplantation after circulatory arrest, the shortage of donors might be significantly alleviated. However, in such non-heart-beating-donors, there is great concern that even a short period of warm ischemia will be deleterious for lung tissue. It was the purpose of this study to look for the efficacy of intrathoracic topical cooling by cold air inside a cadaver after circulatory arrest.
Methods. Ten mongrel dogs were intubated, heparinized and euthanized by potassium chloride injection and inflated with 100% oxygen. In group I (n=5), donor animals were left at room temperature. In group II (n=5), left thoraces of donor animals were cooled by cold gas of dry ice. Intrathoracic, lung surface, and endobronchial temperatures were measured at intervals after death. Two hours later, donor animals were underwent sternotomy, and the lungs were flushed with cold Euro-Collins solution, harvested, and stored inflated in a cold saline solution for two ho
… More
urs. Specimens of donor lungs before transplantation were assessed by histological examination. Then, left lung allo-transplantation was performed. Function of the transplanted left lungs was examined by 10-minute occlusion of the native right bronchus and pulmonary artery while left lung was ventilated with 100% oxygen. Systemic and pulmonary vascular hemodynamics were measured and arterial blood gas was obtained before and during the occlusion.
Results. There was a slow decline in intrathoracic, lung surface, and endobronchial temperatures toward room temperature in group I.In contrast, all three temperatures dropped to approximately 10℃ in 30 minutes in group II.Histological examination of the transplanted lungs demonstrated mild intraalveolar and interstitial edema in group I and almost normal lung structure without frostbite in group II.In group I, all dogs died immediately after the occlusion. In group II, however, three of five dogs survived and showed good lung function.
Conclusions. 1) Intrathoracic topical cooling by cold air inside a cadaver is efficacious in decreasing lung temperature. 2) Transplantation of cadaver lungs harvested in this method might be feasible. Less
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