1999 Fiscal Year Final Research Report Summary
Mechanism of myelopathy and neuronal loss caused, by chronic spinal cord compression in a novel rat model.
| Project/Area Number |
10671314
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Dokkyo University School of Medicine |
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Principal Investigator |
KIM Phyo Dokkyo University School of Medicine, Neurosurgery, Professor and Chairman, 医学部, 教授 (90231290)
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| Co-Investigator(Kenkyū-buntansha) |
KAWAMOTO Toshiki Dokkyo University School of Medicine, Neurosurgery, Instructor, 医学部, 助手 (50301461)
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| Project Period (FY) |
1998 – 1999
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| Keywords | cervical myelopathy / rat model. / spinal cord / compression / motor neuron / cell death |
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| Research Abstract |
A novel experimental model was developed in the rat to reproduce chronic cervical myelopathy. A thin expanding polymer was installed underneath the cervical laminae without causing immediate injury to the spinal cord. After the operation, motor functions were monitored for 25 weeks; voluntary exercise by cumulative count of revolving cages, and locomotion capability by maximal duration on a rotating treadmill.
Immediately after surgery, the two parameters decreased but recovered in two weeks in the compression and in the control (undergoing sham operation) groups. Capability of forced locomotion deteriorated in the compression group with a latency of 17 weeks and progressively thereafter. In the control group, the parameter stayed unchanged throughout 25 weeks. In both groups, amount of voluntary exercise declined gradually in a comparable manner during the observation period.
Motor neurons were counted stereologically in continuous sections (thickness and interval :5 μm, 300 slices) at 1,3,9,and 25 weeks after surgery. The neuron count decreased significantly in 9 weeks (-20.3%) and 25 weeks (-35.5%) after compression. Cross sectional area of the cord was reduced by 11.3 % (25 weeks).
The model successfully reproduces important characteristics of clinical spondylotic myelopathy, namely, progressive course with an insidious onset, and association of motor neuron loss that precedes the symptoms.
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Research Products
(12 results)
