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2000 Fiscal Year Final Research Report Summary

Effect of various cytokines on the metabolism of intervertebral disc cells cultured in vitro

Research Project

Project/Area Number 10671382
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Orthopaedic surgery
Research InstitutionKeio University

Principal Investigator

CHIBA Kazuhiro  Keio University, Orthopaedic Surgery, Assistant Professor, 医学部, 講師 (80179952)

Co-Investigator(Kenkyū-buntansha) IMABAYASHI Hideaki  Keio University, Orthopaedic Surgery, Resident, 医学部, 助手 (40296629)
TSUJI Takashi  Keio University, Orthopaedic Surgery, Resident, 医学部, 助手 (60296639)
Project Period (FY) 1998 – 2000
Keywordsintervertebral disc / nucleus pulposus / annulus fibrosus / cell culture / extracellular matrix / proteoglycan / collagen / cytokine
Research Abstract

Purposes of the current studies were to investigate metabolism of various extracellular matrix molecules of nucleus pulposus (NP) and annulus fibrosus (AF) cells cultured in alginate beads system and its changes after administrations of various various cytokines such as interleukin-1α (IL-1α).
Both NP and AF cells formed two different extracellular matrix pools, cell-associated (CM) and further removed (FRM) components, however, NP cells, have poor ability to retain matrix in CM.AF cells produced more collagen than NP cells. In NP, over 60% of radiolabelled proteoglycans already existed in FRM at initial time point, however less than 40% of that was in FRM of AF at the same time point. Turnover rates of radiolabelled proteoglycans of NP cells in two matrix pools were similar to that of chondrocytes. Radiolabelled proteoglycan in CM of both annulus fibrosus and nucleus pulposus turnover much faster than those in FRM (15 vs>80 days). Treatment with IL-1α caused a significant increase in the rate of proteoglycan turnover in the beads, especially in the CM, of both the NP and AF.
Intervertebral disc cells retain their phenotypical stability and keep producing their specific macromolecule matrix in alginate beads. Acceleration of the rate of PG turnover, particularly in the CM, caused by IL-1α was similar to the age-related changes observed in our previous studies. IL-1α or related substances may play significant role in normal age related processes of intervertebral disc. By identifying metabolic changes occurring in distinct matrix compartments, the alginate bead culture system appears suited to study the effects of cytokines on the metabolism of disc cells. This culture system has applicability to further study the effects of other cytokines and growth factors, the results of which should provide useful information to elucidate possible mechanisms of disc degeneration and may ultimately lead to prevention and reversion of such processes.

  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] K CHIBA, et al: "A new culture system to study the metabolism of the intervertebral disc in vitro"Spine. 23. 1821-1828 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 千葉一裕 他: "椎間板変性の病態生理学"脊椎・脊髄ジャーナル. 12. 471-475 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 千葉一裕 他: "腰椎の臨床-脊椎・椎間板の病態生理"メヂカルビュー社. 321 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Chiba K., et al.: "A new culture system to study the metabolism of the intervertebral disc in vitro"Spine. 23. 1821-1828 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Chiba K., et al.: "Pathophysiology of intervertebral disc degeneration"Spine and Spinal cord. 12. 471-475 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Chiba K., et al.: "Pathophysiology of spine and intervertebral disc In Clinics of the Lumbar Spine"Medical view Inc.. (2001)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2002-03-26  

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