1999 Fiscal Year Final Research Report Summary
Contribution of membrane skeletal protein in osteoblast for bone remodeling
| Project/Area Number |
10671388
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Tokyo Women's Medical University |
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Principal Investigator |
HORIKOSHI Mariko Tokyo Women's Medical University, Dep.of Orthopaedic Surgery, Assistant Professer, 医学部, 講師 (00287400)
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| Co-Investigator(Kenkyū-buntansha) |
INOUE Kazuhiko Tokyo Women's Medical University, Dep.of Orthopaedic Surgery, Professer, 医学部, 教授 (70095024)
TAKAKUWA Yuichi Tokyo Women's Medical University, Dep.of Biochemistry, Professer, 医学部, 教授 (40113740)
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| Project Period (FY) |
1998 – 1999
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| Keywords | bone remodeling / osteoblast / membrane skeleton / protein 4.1 / CD44 / CO44 |
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| Research Abstract |
Remodeling of bone tissue is maintained by bone formation and absorption which are regulated by osteoblasts and osteclasts, respectively. This remodeling is affected by systematic and local factors secreted from these cells as well as cell adhesion. Membrane skeleton has been shown to be involved in secretion of local factors and cell adhesion in other tissues, however, little information is available concerning the skeletal proteins in osteoblasts. In the present experiments we aimed to show the presence and distribution of membrane skeletal proteins in cultured osteoblasts.
Analysis of membrane proteins obtained from mouse cultured osteoblasts (MC3T3-E1) by SDS-PAGE and immunobloting using anti-membrane skeletal proteins such as protein 4.1 as well as transmembrane proteins such as CD44 indicated presence of protein 4.1 and CD44. mRNA of these proteins were identified by analysis of cDNA amplified using RT-PCR of total mRNA obtained from the cultured cells. Immunostaining showed distribution of protein 4.1 in the cytoplasm as well as membranes of these cells. These results were basiccaly confirmed using human cultured osteoblasts (Cryo NHOst). Taken together these relults suggest that the membrane skeletal organization is present in the osteoblasts and that this system may be involved in regulation of secretion and adhesion of osteoblasts.
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