2000 Fiscal Year Final Research Report Summary
The Study of Histomorphometric Measurement, Cytokine Expression and Bone Metabolic Markers Gene Polymorphism in Hemodialysis Patients
| Project/Area Number |
10671389
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Tokyo Women's Medical University |
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Principal Investigator |
KATO Yoshiharu Faculty of Medicine, Tokyo Women's Medical University, 医学部, 助教授 (00143850)
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| Co-Investigator(Kenkyū-buntansha) |
WADA Hiroyoshi Faculty of Medicine, Tokyo Women's Medical University, 医学部, 助手 (80287416)
ITOH Shunichi Faculty of Medicine, Tokyo Women's Medical University, 医学部, 助手 (10307580)
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| Project Period (FY) |
1998 – 2000
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| Keywords | Renal failure / Hemodialysis / Bone histomorphometry / Renal Osteodystrophy / Interleukin-1β / Destructive Spondyloarthropathy / β2-microglobulin / Gene Polymorphism |
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| Research Abstract |
We evaluated 51 chronic renal failure and hemodialysis patients, who had orthopaedic surgery, by bone morphometry, cytokine expression and gene polymorphism. In bone morphometry these patients were histologically classified into three groups ; and osteitis fibrosa type, aplastic type, and osteomalacia type. Osteitis fibrosa demonstrates high turnover bone, and typical characteristic X-ray findings (rugger-jersy appearance etc.). In destructive spondyloarthropathy (DSA) patients aplastic type is more than osteitis fibrosa type and osteomalacia type. In RT-PCR cytokine studies IL- 1 β was clearly expressed in only osteitis fibrosa group (high turnover bone), and not control and other types. This finding indicates that this cytokine induce cell differentiation, growth and activity of bone forming cells in high turnover bone. We postulated that amyloid deposit (β2-microglobulin) may be responsible for the induction of DSA, and spinal osteoporosis may be responsible for the progression of DSA.We examined apoprortein E gene polymorphism type and vitamin D receptor gene polymorphism for gene predisposition of DSA.In DSA patients E3/3 type (72.5%) in apoprortein E gene polymorphism type and bb type (64.7%) in vitamin D receptor gene polymorphism are most frequent than other types, and the patients with E3/3 and bb may be related to DSA onset and progression.
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