1999 Fiscal Year Final Research Report Summary
Role of Nitric Oxide on Minimum Alveolar Anesthetic Concentration
| Project/Area Number |
10671398
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | University of Tsukuba |
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Principal Investigator |
FUKUDA Taeko Institute of Clinical Medicine, University of Tsukuba Assistant Professor, 臨床医学系, 講師 (40228911)
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| Co-Investigator(Kenkyū-buntansha) |
SAITO Shigeyuki Institute of Clinical Medicine, University of Tsukuba Assistant Professor, 臨床医学系, 講師 (20186935)
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| Project Period (FY) |
1998 – 1999
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| Keywords | Nitric Oxide (NO) / Inhalational Anesthetics / MAC / 7-Nitro Indazole (7-NI) / Dexmedetomidine |
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| Research Abstract |
"Effects of 7-Nitro Indazole on Halothane Minimum Alveolar Anesthetic Concentration"
Methods : Fourteen Sprague-Dawley rats were divided into 7-Nitro indazole (7-NI ; selective neuronal nitric oxide synthase inhibitor) group and control group. We measured minimum alveolar anesthetic concentration (MAC) of halothane before and after injection of 7-NI (100 mg/kg) or vehicle. Using NADPH-diaphorase histochemistry, we also investigated the nNOS activity of the locus ceruleus (LC) and the spinal cord (SC).
Results : 7-NI reduced the MAC about 50 %, which was accompanied by about 25 % suppression of nNOS staining in SC and LC.
"Halothane Minimum Alveolar Anesthetic Concentration and NADPH-diaphorase histochemistry after Dexmedetomidine injection"
Methods : Thirteen-six Sprague-Dawley rats were divided into three Dexmedetomidine (DEX) groups (50μg/kg bolus, 50μg/kg/day ; three-day and two-week continuous administration) and three control groups (vehicle). We investigated halothane MAC and the nNOS activity of the LC and the SC using NADPH-diaphorase histochemistry.
Results : Bolus injection of DEX reduced the MAC about 50 %, however, did not change the NADPH-diaphorase histochemistry. On the other hand, continuous administration of DEX did not reduce the MAC, however, nNOS activity of the LC and IV-V laminae of the lumbar SC decreased in the three-day DEX group, but not the two-week DEX group.
Conclusion : Nitric oxide (NO) plays an important role in decision of MAC, however, NO is not only one factor.
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