2001 Fiscal Year Final Research Report Summary
Rapid Detection of Donor and Allograft Transmittable Infection : Using PCR Technique
| Project/Area Number |
10671501
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Urology
|
|---|
| Research Institution | Fujita Health University |
|---|
Principal Investigator |
HOSHINAGA Kiyotaka Fujita Health Univ. Urology, Professor, 医学部, 教授 (30229174)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
ISHIKAWA Kiyohito Fujita Health Univ. Urology, Assistant Professor, 医学部, 講師 (80312114)
SHIROKI Ryoichi Fujita Health Univ. Urology, Associate Professor, 医学部, 助教授 (70226330)
|
|---|
| Project Period (FY) |
1998 – 2001
|
| Keywords | Cadaveric Doner / Renal Transplant / Bacteria / Candida / MRSA / Contamination / PCR / Contamination |
|---|
| Research Abstract |
Polymerase chain reaction (PCR) and Rapid shaking culture (RSC) were applied to blood samples obtained from 84 possible donors, the preservation solution (PS) and renal pelvic urine (PU) obtained from 180 grafts (159 grafts were harvested in our center and 21 grafts were transferred from other center). In PCR, appropriate primers were used to detect following genes, fem A: specific to S. aureus mec A: specific to methicillin resistant Staphyrococci, Gyr A: specific to P.aeruginosa and 18S-rDNA: specific to most of the clinically critical fungi. It takes for 4 to 6 hours. RSC was started in parallel with PCR and the result was judged at 6 to 24 hours. The average of total hospitalized period of donors was 9.3 days and the average values of inflammatory indices such as peripheral white blood cell counts and serum C reactive protein were elevated up to 21060/ul and 23.9mg/dl, respectively. Employing PCR and RSC, systemic bacterial infections were detected in 3 possible donors (2 MRSA and 1 bacteroides), and the procurements were cancelled. In 159 grafts harvested in our center, seven grafts were discarded due to the diagnosis of contamination with MRSA (2 grafts) or fungi (5 grafts). In 21grafts transferred from other centers, 2 grafts were discarded diagnosed as MRSA or Candida infection. One hundred sixty four grafts except 7 grafts discarded due to other reasons were finally transplanted. Following transplants, no single infection was transmitted.
|
