2000 Fiscal Year Final Research Report Summary
Regulation of prostatic growth and differentiation : Interaction between androgen and growth factors.
| Project/Area Number |
10671508
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | AICHI CANCER CENTER |
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Principal Investigator |
SUGIMURA Yoshiki AICHI CANCER CENTER, RESEARCH FELLOW, 研究所, 研究員 (90179151)
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| Co-Investigator(Kenkyū-buntansha) |
TATEMATSU Masae DEPARTMENTNT OF PATHOLOGY, AICHI CANCER CENTER, DIRECTOR, 病理学第一部, 部長 (70117836)
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| Project Period (FY) |
1998 – 2000
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| Keywords | prostate / organ culture / estorogen / TGF-α / androgen / TGF-α / 偏平上皮化成 / 増殖因子 |
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| Research Abstract |
In the present study a serum-free organ culture system was used to examine the effects of estradiol and TGF-α on adult mouse dorsolateral (DLP) ducts. DLP was microdissected from adult male Balb/c mice and placed into floating serum-free culture. Medium was supplemented with either estradiol (E2, 10^<-9>M) or TGF-α (20ng/ml). Control cultures were either unsupplemented or received testosterone (T, 10^<-9>M). DLP cultures grown in unsupplemented medium underwent regression similar to that seen in vivo following castration. DLPs grown in the presence of T maintained a typical prostatic differentiation and exhibited extremely low levels of either proliferative activity or cell death. DLPs grown in the presence of either E2 or TGF-α underwent rapid epithelial proliferation characterized by incorporation of bromodeoxyuridiine (BrdU) in the basal epithelial layer. 4 days after the initiation of culture basal cell hyperplasia was evident characterized by the presence of multiple layers of CK 14-expressing cells and some expression of CK 10 could also be detected. Following 6 days of culture CK 10 expression was pronounced in many of the prostatic ducts. Squamous metaplasia is characterized by rapid epithelial proliferation leading to the production of a mass of epithelial cells expressing the differentiated squamous cell cytokeratins (CKs) 1 and 10 as compared to the normal luminal prostatic cell CKs 8 and 18. These data demonstrate that TGF-α and E2 elicit similar responses in the adult mouse prostate in vitro and suggest a possible role for TGF-α as a mediator of squamous metaplasia in the prostate.
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[Publications] Lipschutz, H.J., Fukami, Y., Yamamoto, M., Tatematsu, M., Sugimura, Y., Kusakabe, M., Cunha, G.R.: "Clonality of urogenital organs as determined by analysis of chimeric mice."Cells Tissues Organs. 165 (2). 57-66 (1999)
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「研究成果報告書概要(欧文)」より
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[Publications] Ishii, K., Usui, S., Sugimura, Y., Yoshida, S., Hioki, T., Tatematsu, M., Yamamoto, H.and Hirano, K.: "Aminopeptidase regulation by zinc in human prostate participates in tumor cell in vasion."Int J of Cancer. 9249-9254 (2001)
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「研究成果報告書概要(欧文)」より
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[Publications] Ishii, K., Usuii, S., Sugimura, Y., Yamamoto, H., Yoshikawa, K.and Hirano, K.: "Extract from Serenoa repens suppresses the invasion activity of humanurological cancer cells by inhibiting urokinase-type plasminogen activator"Biol. Pharm. Bull.. 24. 226-230 (2001)
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「研究成果報告書概要(欧文)」より
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[Publications] Ishii, K., Usui, S., Yamamoto, H., Sugimura, Y., Tatematsu, M., and Hirano, K.: "Decrease of Metallothionein and Aminopeptidase N in Renal Cancer Tissues."J of Biochem.. 129. 253-258 (2001)
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「研究成果報告書概要(欧文)」より
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[Publications] Ishii, K., Usui, S., Sugimura, Y., Yamamoto, H., Yoshikawa, K.and Hirano, K.: "Inhibition of Aminopeptidase N (AP-N) and Urokinase-Type Plasminogen Activator (uPA) by Zinc Leads to the Suppression of Invasion Activity in Human Urological Cancer Cells."Biol. Pharm. Bull.. 24226-24230 (2001)
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「研究成果報告書概要(欧文)」より
