2001 Fiscal Year Final Research Report Summary
Regulation of prostatic growth and differentiation : Interaction between androgen and growth factors
| Project/Area Number |
10671508
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | AICHI CANCER CENTER |
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Principal Investigator |
SUGIMURA Yoshiki AICHI CANCER CENTER, RESEARCH FELLOW, 研究所, 研究員 (90179151)
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| Co-Investigator(Kenkyū-buntansha) |
TATEMATSU Masae DEPARTMENT OF PATHOLOGY, AICHI CANCER CENTER, DIRECTOR, 病理学第一部, 部長 (70117836)
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| Project Period (FY) |
1998 – 2000
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| Keywords | prostate / organ culture / estorogen / TGF-α / androgen / TGF-α / 扁平上皮化成 / 増殖因子 |
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| Research Abstract |
In the present study a serum-free organ culture system was used to examine the effects of estradiol and TGF-α on adult mouse dorsolateral (DLP) ducts. DLP was microdissected from adult male Balb/c mice and placed into floating serum-free culture. Medium was supplemented with either estradiol (E2, 10^< 9>M) or TGF-α (20ng/ml). Control cultures were either unsupplemented or received testosterone (T, 10^<-9>M). DLP cultures grown in unsupplemented medium underwent regression similar to that seen in vivo following castration. DLPs grown in the presence of T maintained a typical prostatic differentiation and exhibited extremely low levels of either proliferative activity or cell death. DLPs grown in the presence of either E2 or TGF-α underwent rapid epithelial proliferation characterized by incorporation of bromodeoxyuridine (BrdU) in the basal epithelial layer. 4 days after the initiation of culture basal cell hyperplasia was evident characterized by the presence of multiple layers of CK 14 expressing cells and some expression of CK 10 could also be detected. Following 6 days of culture CK 10 expression was pronounced in many of the prostatic ducts. Squamous metaplasia is characterized by rapid epithelial proliferation leading to the production of a mass of epithelial cells expressing the differentiated squamous cell cytokeratins (CKs) 1 and 10 as compared to the normal luminal prostatic cell CKs 8 and 18. These data demonstrate that TGF- and E2 elicit similar responses in the adult mouse prostate in vitro and suggest a possible role for TGF-α as a mediator of squamous metaplasia in the prostate.
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[Publications] Lipschutz, H.J., Fukami, Y., Yamamoto, M., Tatematsu, M., Sugimura, Y., Kusakabe, M., Cunha, G.R.: "Clonality of urogenital organs as determined by analysis of chimeric mice"Cells Tissues Organs. 165 (2). 57-66 (1999)
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「研究成果報告書概要(欧文)」より
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[Publications] Ishii, K., Usui, S., Sugimura, Y., Yoshida, S., Hioki, T., Tatematsu, M., Yamamoto, H., and Hirano, K.: "Aminopeptidase N regulation by zinc in human prostate participates in tumor cell in vasion"Int J of Cancer. 9249-54 (2001)
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「研究成果報告書概要(欧文)」より
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[Publications] Ishii, K., Usui, S., Sugimura, Y., Yamamoto, H., Yoshikawa, K. and Hirano, K.: "Extract from Serenoa repens suppresses the invasion activity of humanurological cancer cells by inhibiting urokinase-type plasminogen activator"Biol. Pharm. Bull.. 24. 226-230 (2001)
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「研究成果報告書概要(欧文)」より
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[Publications] Ishii, K., Usui, S., Yamamoto, H., Sugimura, Y., Tatematsu, M. and Hirano, K.: "Decrease of Metallothionein and Aminopeptidase N in Renal Cancer Tissues"J of Biochem. 129. 253-258 (2001)
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「研究成果報告書概要(欧文)」より
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[Publications] Ishii, K., Usui, S., Sugimura, Y., Yamamoto, H., Yoshikawa, K. and Hirano, K.: "Inhibition of Aminopeptidase N (AP-N) and Urokinase-Type Plasminogen Activator (Upa) by Zinc Leads to the Suppression of Invasion Activity in Human Urological Cancer Cells"Biol. Pharm. Bull.. 24. 226-230 (2001)
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「研究成果報告書概要(欧文)」より
