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2000 Fiscal Year Final Research Report Summary

Analysis of the X and Y Chromosomes in Human Spermatozoa Prepared by Swim-up and Percoll Technique

Research Project

Project/Area Number 10671578
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Obstetrics and gynecology
Research InstitutionDepartment of Obstetrics & Gynecology, Kinki University School of Medicine

Principal Investigator

HOSHIAI Hirohsi  Kinki University School of Medicine, professor, 医学部, 教授 (50111290)

Project Period (FY) 1998 – 2000
Keywordssperm / Swim-up / Percoll / FISH / aneuploidy
Research Abstract

This study has detected whether the selection of motile sperm by Swim-up and Percoll method can fully reflect true genetic quality. We used the twocolor fluorescence in situ hybridization (FISH) technique to simul taneously identify X- and Y-bearing sperm before and after the Swim-up or the Percoll. The samples were ob-tained by masturbation, and prepared for the swim-up and Percoll procedures. Neat semen was served as the control. A total of 21843 sperm in the neat semen (control) were scored. The percentage of Xbearing sperm : Y-bearing sperm was 50.0% : 49.8%, and aneuploidy sperm 0+26%. In the Swirnmp upper layer, 15289 sperm were scored and X : Y sperm was 47.6% : 52.2%, aneuploidy sperm 0.17〜. In the swim-up bottom layer, 20185 sperm were scored and X : Y sperm was 52.2% : 47.3〜, aneuploidy sperm 0.50%. And in the Percoll group 18536 sperm were scored and X : Y sperm was 50.2% : 49.6%, aneuploidy sperm 0.19〜. A significant difference was observed in the swirn, up upper layer fraction and Swirnrup bottom layer fraction (p<0.05). About 3C) 40% of the aneuploidy sperm were removed by the swim-up or the Percoll procedures, but there was about 0.2% aneuploidy sperm existed yet. We should combine certain procedures to obtain true normal sperm for assisted reproductive tech-niques.

  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] 李萍,高瀬規久也,三橋洋治,星合昊: "乏精子症、精子無力症の染色体異常の検討"産婦人科の実際. 48(8). 1135-1138 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ping Li,Hiroshi Hoshiai;: "Detection of Numerical Chromosome Abnormalities in Human Spermatozoa by Three-Color fluorescence in situ Hybridization,"Obstetrics & Gynecology Research. Vol.24(6). 385-392, (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 李萍,三橋洋治,高瀬規久也: "向林学,星合昊FISH法による各種精子調整法回収精子の性染色体異常の検討;"日本不妊学会雑誌、. 第43巻第3号. 243-247, (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 李萍,高瀬規久也,向林学: "三橋洋治,星合昊;男性不妊治療における補中益気湯の有効性について、"産婦人科漢方研究のあゆみ. 第14巻1号. 126-129、 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 李萍,高瀬規久也,向林学,高島忠守,三橋洋治,星合昊: "男性不妊における補中益気湯の臨床効果について、"産婦人科の進歩、. 第48巻4号. 406-410、 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ping Li, Hiroshi Hoshiai: "Detection of Numerical Chromosome Abnormalities in Human Spermatozoa by Three-Color fluorescence in situ Hybridization."Obstetrics & Gynecology Research. Vol.24 (6). 385-392 (1998)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2002-03-26  

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