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1999 Fiscal Year Final Research Report Summary

Experimental Study of ZAPRINAST on Aqueous Outflow Mechanism

Research Project

Project/Area Number 10671645
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Ophthalmology
Research InstitutionYamaguchi University

Principal Investigator

SUZUKI Ryo  Yamaguchi University, School of Medicine, Associate Professor, 医学部, 助教授 (50163027)

Co-Investigator(Kenkyū-buntansha) FUJIKURA Yoshihisa  Oita Medical University, School of Medicine, Professor, 医学部, 教授 (10165368)
Project Period (FY) 1998 – 1999
Keywordsoutflow facility / phosphodiesterase inhibitor / glaucoma / trabecular meshwork / intraocular muscle
Research Abstract

We designed our study to find a method to understand a clue for curing glaucoma. We found that zaprinast, a specific inhibitor of phosphodiesterase type 5, increased the outflow facility of enucleated bovine eyes. Since the drug increases cGMP, it was a special concern for us to study the effect of zaprinast on intraocular muscles, outflow facility and shape of cultured trabecular meshwork cells.
1 Results (1998-1999)
(1) We structured eye-perfusion system.
Zaprinast markedly increased the outflow facility by more than 200%. On the other hand, dB-cAMP changed the outflow facility only to some extent by 95.1±4.9, 78.8±4.9, 80.9±4.2%, respectively.
(2) Zaprinast did not induce miosis or contraction of bovine ciliary muscle.
Zaprinast did not alter the tone of the intraocular muscles. However, when the muscle was precontracted by carbachol, zaprinast (0.1 to 100 μM) relaxed the muscle.
(3) Zaprinast and/or sildenafil increased the outflow facility of the bovine eye.
Zaprinast had increased the outflow facility much more than sildenafil did.
(4) Cell culture study of trabecular meshwork cells and ciliary muscles :
Zaprinast altered the cell shape of trabecular meshwork cells. Zaprinast contracted the cell within 2 hours, and sildenafil in 5 hours.
We performed the perfusion study, muscle- and cell cuture- studies to learn the effect of zaprinast to cure glaucoma.
Zaprinast may be a candidate of glaucoma drug in future, because the drug could increase the outflow facility of the eye and did not contract the ciliary muscle, and the effect was much greater than ever reported.
2 Matters which we changed from the initial research planning (fees)
We initially planned to go abroad to communicate and make a presentation of the present study. Unfortunately, however, consumption supplies were insufficient and gave up the planned idea to go abroad. Fixtures were purchased as initially designed.

  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Suzuki R: "Bull's eye macular dystrophy associated with periheral involvement"Ophthalmologica. 212. 260-267 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nomura S: "Three types of massive and prolonged retinal bleeding as a complication of hypotony after trabeculectomy"Ophthalmologica. 231. 135-138 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nomura S: "Optic glioma with characteristic bilateral optic atrophy in a 3-year-old girl"Pediatric Neurosurg. 31. 213-218 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Suzuki R: "Myocilin gene abnormality in patients with open-engle glaucoma and noemal tension glaucoma with a family history"Br J Ophthalmol. (印刷中).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 鈴木 亮: "高眼圧をひきおこす内服薬について"あたらしい眼科. 16. 71-75 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 鈴木 亮: "緑内障の早期手術の是非について(参考)"あたらしい眼科. 16. 179-182 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 鈴木 亮: "(瞳孔反応異常)「眼科診療便利手帳」"診断と治療社. 2 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Suzuki R.: "Bull's eye macular dystrophy associated with peripheral involvement."Ophthalmologica. 212. 260-267 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Suzuki R.: "Three types of massive and prolonged retinal bleeding as a complication of hypotony after trabeculectomy."Ophthalmologica. 213. 135-138 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nomura S.: "Optic glioma with characteristic bilateral optic atrophy in a 3-year-old girl."Pediatr Neurosurg. 31. 213-218 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Suzuki R.: "Myocilin gene abnormality in patients with open-angle glaucoma and normal tension glaucoma with a family history."(in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Suzuki R.: "Medical drugs that induce on intraocular pressures."Atarashii Gannka. 16. 71-75 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Suzuki R.: "When should we perform glaucoma surgery?"Atarashii Gannka. 16. 179-182 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Suzuki R.: "Pupil Response Abnormality. In Ophthalmic Diagnosis Book."Sinndann To Chiryousha, Co.. (1998)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2001-10-23  

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