Experimental Study of the Promoting Agents for Osteo- and Chondro- induction by Bone Morphogenetic Protein

1999 Fiscal Year Final Research Report Summary

• Project/Area Number: 10671691
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plastic surgery
• Research Institution: Kansai Medical University
• Principal Investigator: KUSUMOTO Kenji K.M. Univ., Dept Plast Surg, Assoc Prof., 医学部, 助教授 (20161630)
• Co-Investigator(Kenkyū-buntansha): AKIOKA Jiro K.M. Univ., Dept Plast Surg, Instructor, 医学部, 助手 (20288799) ; OGAWA Yutaka K.M. Univ., Dept Plast Surg, Prof., 医学部, 教授 (70026912)
• Project Period (FY): 1998 – 1999
• Keywords: Bone morphogenetic protein / osteoinduction / carrier / atelopeptide type I collagen / osteogenesis / skeletal reconstruction / 骨再建

Research Abstract

Heterotopic osteoinduction ability of bone morphogenetic protein (BMP) is much anticipated for skeletal reconstruction. Concerning the clinical application of BMP, various promoting agents on the osteoinduction by rhBMP-2 were studied. Those agents were prostaglandin E1, E2, cAMP, calcitonine, K2, O2 and blood supply.
The phalmachological dosage of these agents promoted the osteoinduction. However, high dosage of the agents suppressed the osteogenesis. Those studies showed that each agent acted on the various phases of osteogenesis.
These results suggested adequate dosages of agents in clinical usage of BMP.

Research Products

• [Publications] Kusumoto K, Bessho K, Fujimura K, Akioka J, Oguwa Y, Iizuka T: "Prefabricated muscle flap including bone induced by recombinant human BMP-2 ; an experimental study of ectopic osteoinduction in a rat latissimus dorsi muscle flap"Brit J Plast Surg. 51. 275-281 (1998)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Yoshida K, Bessho K, Fujimura K, Konishi Y, Kusumoto K, Ogawa Y, Iizuka T: "Enhancement by recombinant human bone morphogenetic protein-2 of bone formation by means of porous hydroxyapatite in mandibular bone defects"J Dent Res. 78(9). 1505-10 (1999)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Kiyozuka Y, Miyazaki H, Yoshizawa K, Senzaki H Yamamoto D, Inoue K, Bessho K, Okubo Y, Kusumoto K, Tsubura A: "An autopsy case of malignant mesothelioma with osseous and cartilagious differentiation : Bone morphoganetic protein-2 in mesothelial cells and its tumor"Digest Dis Sci. 44. 1626-31 (1999)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Okubo Y, Bessho K, Fujimura K, Konishi Y, Kusumoto K, Ogawa Y, Iisuka T: "Osteoinduction by recombinant human bone morphogenetic protein-2 at intramuscular, intermuscular, subcutaneous and intrafatty sites"Int J Oral Maxillofac Surg. 29. 62-66 (2000)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Okubo Y, Bessho K, Fujimura K, Kusumoto K, Ogawa Y, Iizuka T: "Effect of elcatnin (Carbocalcitonin) on osteoinduction by recombinant human bone morphogenetic protein-2"Biochem Biophys Res Commun. (in press).
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Okubo Y, Bessho K, Fujimura K, Konishi Y, Kusumoto K, Ogawa Y, Iizuka T: "Osteogenesis by recombinant human bone morphogenetic protein-2 at skeletal sites"Clinic Orthop Relat Res. (in press).
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Kusumoto K and Bessho K: "Jaw reconstruction with BMP, collagen and hydroxyapatite in vivo"In T. Sam Lindoholm (ed.) : The new book on bone morphogenetic protein, R.G.Landes company, Austin, Texas (in press).
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Yoshida K, Bessho K, Fujimura K, Kusumoto K, Ogawa Y, Tani Y, Iizuka T: "Osteoinduction capability of recombinant human bone morphogenetic protein-2 in intramuscular and subcutaneous sites : an experimental study."J Cranio-Maxillofac Surg. 26. 112-115 (1998)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Kusumoto K, Bessho K, Fujimura K, Akioka J, Ogawa Y, Iizuka T: "Prefabricated muscle flap including bone induced by recombinant human BMP-2 ; an experimental study of ectopic osteoinduction in a rat latissimus dorsi muscle flap."Brit J Plast Surg. 51(4). 275-280 (1998)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Kusumoto K, Bessho K, Fujimura K, Akioka J, Okubo Y, Ogawa Y, Iizuka T: "Prefabricated muscle flap with bony tissue ; an experiemental study of heterotopic osteoinduction by a combination of rhBMP-2, cillagen and porous hydroxyapatite wrapped with a muscle flap."J Cranio-Maxillofac Surg. 26(Supple 1). 101 (1998)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Bessho K, Kusumoto, K, Fujimura K, Konishi Y, Ogawa Y, Iizuka T: "Comparison of recombinant and purified human bone morphogenetic protein."Brit J Oral maxillofac Surg. 37. 2-5 (1999)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Okubo Y, Bessho K, Fujimura K, Kusumoto K, Ogawa Y, Tani Y, Iizuka T: "Comparative study of intramuscular and intraskeletal osteogenesis by recombinant human bone morphogenetic protein-2."Oral Surg Oral Med Oral Pathol Oral Radio Endod. 87(1). 34-38 (1999)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Yoshida K, Bessho K, Fujimura K, Konishi Y, Kusumoto K, Ogawa Y, Iizuka T: "Enhancement by recombinant human bone morphogenetic protein-2 of bone formation by means of porous hydroxyapatite in mandibular bone defects."J Dent Res. 78(9). 1505-10 (1999)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Kiyozuka Y, Miyazaki H, Yoshizawa K, Senzaki H, Yamamoto D, Inoue K, Bessho K, Okubo Y, Kusumoto K, Tsubura A: "An autopsy case of malignant mesothelioma with osseous and cartilagious differentiation : Bone morphogenetic protein-2 in mesothelial cells and its tumor."Digest Dis Sci. 44. 1626-31 (1999)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Okubo Y, Bessho K, Fujimura K, Konishi Y, Kusumoto K, Ogawa Y, Iizuka T: "Osteoinduction by recombinant human bone morphogenetic protein-2 at intramuscular, intermuscular, subcutaneous and intrafatty sites."Int J Oral Maxillofac Surg. 29. 62-66 (2000)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Okubo Y, Bessho K, Fujimura K, Kusumoto K, Ogawa Y, Iizuka T: "Effect of elcatnin (Carbocalcitonin) on osteoinduciton by recombinant human bone morphogenetic protein-2."Biochem Biophys Res Commun. (in press.).
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Okubo Y, Bessho K, Fujimura K, Konishi Y, Kusumoto K, Ogawa Y, Iizuka T: "Osteogenesis by recombinant human bone morphogenetic protein-2 at skeletal sites."Clinic Orthop Relat Res. (in press.).
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Kusumoto K and Bessho K: "Jaw reconstruction with BMP, collagen and hydroxyapatite in vivo. In T. Sam Lindoholm (ed.) : The new book on bone morphogenetic protein"R. G. Landes company, Austin, Texas.. (in press.)
  • Description: 「研究成果報告書概要(欧文)」より