Purification and molecular basis analysis of IL-1β like substance form periodontopathic bacteria.

2000 Fiscal Year Final Research Report Summary

• Project/Area Number: 10671720
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Morphological basic dentistry
• Research Institution: Meikai University (2000); Nihon University (1998-1999)
• Principal Investigator: OCHIAI Kuniyasu Meikai University, School of Dentistry, Professor., 歯学部, 教授 (50095444)
• Co-Investigator(Kenkyū-buntansha): KURITA-OCHIAI Tomoko Nihon University, School of Dentistry at Matsudo, Assistant professor., 松戸歯学部, 講師 (20130594)
• Project Period (FY): 1998 – 2000
• Keywords: IL-1β / periodontopathic bacteria / Actinobacills / Prevotella / Porphyromonas

Research Abstract

Interleukin-1β (IL-1β) is a polypeptide cytokine that mediates many physiological to infection and inflamation and is a growth factor for certain mamalian cells. We revealed that certain periodontopathic bacteria produce unknown substance which react with mouse anti-human IL-1β antibody. Therefore, we examined the poduction of IL-1β like substance by periodontopathic bacteria, such as Actinobacills, Prevotella, Porphyromonas, Capnocytophaga, Fusobacterium. ELISA results clearly revealed that Actinobacills actinomycetemcomitans, Prevotella intermedia and Prevotella nigrescence produce IL-1β like substance. Amino acid analysis from N-terminal of affinity purified this substances were not a simillar as human IL-1β. The differensis were, also, foud form the analysis of RT-PCR products. Further investigation is required in antigenic site on IL-1β like substance.