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2000 Fiscal Year Final Research Report Summary

Alterations of neuronal response and transcription factors in the trigeminal nucleus following deafferentation pain

Research Project

Project/Area Number 10671738
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Functional basic dentistry
Research InstitutionOsaka University

Principal Investigator

YONEHARA Norifumi  Graduate School of Dentistry Osaka University Associate Professor, 大学院・歯学研究科, 助教授 (70124534)

Co-Investigator(Kenkyū-buntansha) KAKU Tetsuki  Graduate School of Medicine Osaka University Associate Professor, 大学院・医学系研究科, 助教授 (50126570)
TAKEMURA Motohide  Graduate School of Dentistry Osaka University Assistant Professor, 大学院・歯学研究科, 講師 (70192169)
Project Period (FY) 1998 – 2000
KeywordsDeafferentation pain / Allodynia / Trigeminal nucleus caudalis / Nitric oxide / Inferior alveolar nerve / NMDA
Research Abstract

This study has been conducted to investigate the mechanisms of development of deafferentation pain including allodynia and hyperalgesia. (1) Neuropathic surgery for animal model : The induction of deafferentation pain was achieved by extraction of the left upper and lower incisor teeth or loose ligation of the inferior alveolar nerve with a chromic gut suture. Mechanical stimulation was applied within the surface of skin around whisker and cheek, sensory of which is controlled by the trigeminal nerves. As an indicator of deafferentation pain, the development of allodynia was determined by von Frey filaments. Tactile allodynia developed by day 7 after the nerve injury and lasted for at least 3 weeks. (2) Mechanisms on development of tactile allodynia : (1) Behavioral experiment : Tactile allodynia was recovered by administration of carbamazepine, N^G-monomethyl-L-arginine (L-NMMA ; nitric oxide (NO) synthase inhibitor) and MK-801 in dosedependent manner. (2) Electrophysiological experiment : The alteration of NO current evoked by the i.v. administration of N-methyl-D-aspartate (NMDA) and MK801 was significantly larger in the ipsilateral side of the superficial layers of the trigeminal nucleus caudalis of operated rats than that of control animals. (3) Immunohistochemical experiment : The number of NO synthase (NOS)-positive neurons were measured in the trigeminal nucleus caudalis. The number of nNOS-positive neurons increased in the layers I-II and III-IV in both side (ipsilateral and contralateral) of operated animals. These results suggest that allodynia developed following dental peripheral nerve injury, and NMDA/NO pathways may be involved in development of tactile allodynia. Crrently we investigate what signal transduction pathways and transcription factors are involved in development of such tactile allodynia. (259 words)

  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] Takagi,J. et al.: "Serotonin receptor subtypes involved in modulation of electrical acupuncture"Jpn.J.Pharmacol.. 78. 511-514 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yonehara,N. et al.: "Involvement of nitric oxide in neurogenic inflammation"The Biology of Nitric Oxide, Part 6 Portland Press, London, (Moncada,S. et al eds.). 208 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yonehara,N. et al: "Effect of nitric oxide on substance P release from the peripheral endings of primary afferent neurons"Neurosci.Letters. 271. 199-201 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yonehara,N. et al: "Interaction between nitric oxide and substance P on neat-induced inflammation in rat paw"Neurosci.Res.. 36. 35-43 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshimura,M. et al: "Effects of topically applied capsaicin cream on neurogenic inflammation and thermal sensitivity"Jpn.J.Pharmacol.. 82. 116-121 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yonehara,N. et al.: "Influence of painful chronic neuropathy on neurogenic inflammation"Pain. (in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takagi, J.and Yonehara, N.: "Serotonin receptor subtypes involved in modulation of electrical acupuncture."Jpn.J.Pharmacol.. 78. 511-514 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yonehara, N.and Yoshimura, M.: "Effect of nitric oxide on substance P release from the peripheral endings of primary afferent neurons."Neuroscience Letters. 271. 199-201 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yonehara, N.and Yoshimura, M.: "Interaction between nitric oxide and substance P on heat-induced inflammation in rat paw."Neurosci.Res.. 36. 35-43 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshimura, M., Yonehara, N., Ito, T., Kawai, Y.and Tamura, T.: "Effects of topically applied capsaicin cream on neurogenic inflammation and thermal sensitivity"Jpn.J.Pharmacol.. 82. 116-121 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yonehara, N.and Yoshimura, M.: "Influence of painful chronic neuropathy on neurogenic inflammation"Pain. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yonehara, N.: "Influence of serotonin receptor antagonists on substance P and serotonin release evoked by tooth pulp stimulation with electro-acupuncture in the trigeminal nucleus caudalis of the rabbit."Neurosci.Res.. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yonehara, N.and Yoshimura, M.: "Involvement of nitric oxide in neurogenic inflammation."Portland Press, London, The Biology of Nitric Oxide Part 6 (Moncada, S., Toda, N., Maeda, H.and Higgs, E.A., editors). 208 (1998)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2002-03-26  

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