2000 Fiscal Year Final Research Report Summary
The role of neuromodulators on the secretion of fluid and protein from salivary gland cells.
| Project/Area Number |
10671755
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional basic dentistry
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| Research Institution | The Nippon Dental University |
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Principal Investigator |
IWABUCHI Yoshiki The Nippon Dental University at Niigata Department of Dental Pharmacology Associate Professor, 新潟歯学部, 助教授 (80095067)
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| Project Period (FY) |
1998 – 2000
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| Keywords | Submandibular Gland / Fluid Secretion / Protein Secretion / Neuropeatide Y / VIP / Substance P / SNI-2011 / Pilocarpine |
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| Research Abstract |
This study was carried out to elucidate the role of neuromodulators on the secretion of fluid and protein from salivary gland cells. 1 : Effects of neuropeptide Y on the secretion of fluid and protein from rat submandibular gland induced by the combination of substance P and VIP (vasoactive intestinal peptide) : (1) Substance P elicited large volumes of saliva whereas neuropeptide Y and VIP did not elicit saliva. (2) VIP potentiated the substance P-mediated secretion of fluid and protein. (3) Neuropeptide Y did not affect SP-mediated secretion of fluid. (4) The potentiating effect of secretion of fluid and protein and the long lasting effect of fluid was reduced by neuropeptide Y in a dose- dependent manner. 2 : Subtypes of adrenergic and muscarinic receptors mediating secretion of fluid and protein from rat submandibular gland induced by SNI-2011 and pilocarpine. (1) Salivation on early stage after administration of SNI-2011 and pilocarpine caused through stimulation of muscarinic M_1 receptors in the superior cervical ganelion. (2) Sialogogic effect induced by stimulation of muscarinic M_1 receptors was higher pilocarpine than SNI-2011. (3) Sialogogic action on last stage after administration of SNI-2011 involves stimulation of M_3 receptors and its action was higher than pilocarpine. (4) SNI-2011 and pilocarpine-mediated secretion of fluid was enhanced by VIP.These results sugest that neuropeptide Y and VIP act as neuromodulator on neurotransmitter, tachykinin and muscarinic agents-mediated secretion of fluid and protein from salivary gland.
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