2000 Fiscal Year Final Research Report Summary
Regulatory mechanism for gene expression during the development of mouse masseter muscle
| Project/Area Number |
10671757
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional basic dentistry
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| Research Institution | Tsurumi University |
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Principal Investigator |
YAMANE Akira Dental Medicine, Tsurumi University Assistant, 歯学部, 助手 (20166763)
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| Co-Investigator(Kenkyū-buntansha) |
OHNUKI Yoshiki Dental Medicine, Tsurumi University Assistant, 歯学部, 助手 (50288114)
SAEKI Yasutake Dental Medicine, Tsurumi University Assistant Prof., 歯学部, 助教授 (20046113)
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| Project Period (FY) |
1998 – 2000
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| Keywords | rat / masseter muscle / acetylcholine receptor / δ subunit / ε subunit / γ subunit / competitive PCR / development |
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| Research Abstract |
There are no published studies on synaptogenesis focusing on the elimination of the nicotinic acetylcholine receptor (nAChR) outside the neuromuscular junction and the nAChR subunit switch from the embryonic-type (α_2βγδ subunits) to the adult-type (α_2βεδ subunits). We analyzed the expression level of δ subunit mRNA, which was present through development, to identify the time course of the nAChR elimination, and the expression level of the ε and γ subunit mRNAs to identify the time course of the nAChR subunit switch in the rat masseter muscle. Rats from 0 to 72 days of age were used, kept in the same cages as their mothers by 18 days of age and then fed a pellet diet thereafter. The mRNA amounts were measured by competitive polymerase chain reaction in combination with reverse transcription. The expression profile of these subunit mRNAs in that rat masseter muscle indicated that the nAChR was mainly eliminated between 7 and 28 days of age and that the nAChR mostly switches from the embryonic to adult types between 1 and 21 days of age. Since the rat feeding behavior seems to switch from suckling to biting after 19 days of age and masseter muscle is known to play an important role in the biting movement of jaw, the completion of the nAChR elimination and switch in the masseter muscle may be related to the switch of the feeding behavior during the postnatal development.
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Research Products
(28 results)
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[Publications] TAKAHASHI,K., YAMANE,A., BRINGAS,P.JR., CATON,J., SLAVKIN,H.C.and ZEICHNER-DAVID,M.: "Induction of amelogenin and ameloblastin by insulin and insulin-like growth factors (IGF-I and IGF-II) during embryonic mouse tooth development in vitro"Connect Tissue Res.. 38(1-4). 269-278 (1998)
Description
「研究成果報告書概要(欧文)」より
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[Publications] YAMANE,A., BRINGAS,P.Jr., MAYO,M.L., AMANO,O, TAKAHASHI,K., VO,H., SHUM,L.and SLAVKIN,H.C.: "Transforming growth factor alpha up-regulates desmin expression during embryonic mouse tongue myogenesis"Dev.Dyn.. 213(1). 71-78 (1998)
Description
「研究成果報告書概要(欧文)」より
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[Publications] YAMANE,A., TAKAHASHI,K., MAYO,M., VO,H., SHUM,L., ZEICHNER-DAVID,M.and SLAVKIN,H.C.: "Induced expression of MyoD, myogenin and desmin during myoblast differentiation in embryonic mouse tongue development"Archs oral Biol.. 43(5). 407-416 (1998)
Description
「研究成果報告書概要(欧文)」より
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[Publications] AMANO,O., BRINGAS,P., TAKAHASHI,I., TAKAHASHI,K., YAMANE,A., CHAI,Y., NUCKOLIS,G.H., SHUM,L.and SLAVKIN,H.C.: "Nerve growth factor (NGF) supports tooth morphogenesis in mouse first branchial arch explants"Dev.Dyn.. 216(4). 299-310 (1999)
Description
「研究成果報告書概要(欧文)」より
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[Publications] YAMANE,A., OHNUKI,Y., SAEKI,Y., YANAGISAWA,K.and CHIBA,M.: "Expression of IGFs, IGF receptors myoD family and acetylcholine receptors during the postnatal development of rat skeletal muscle"In Neurobiology of mastication-From molecular to systems approach (Eds.Nakamura, Y.and Sessle, B.J.), Elsevier, Amsterdam. 89-92 (1999)
Description
「研究成果報告書概要(欧文)」より
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[Publications] OHNUKI,Y., YAMANE,A., SAEKI,Y.and YANAGISAWA,K.: "Effects of bite-opening treatment on the myosin heavy chain expression in rat masseter muscle"In Neurobiology of mastication-From molecular to systems approach (Eds.Nakamura, Y.and Sessle, B.J.), Elsevier, Amsterdam. 411-413 (1999)
Description
「研究成果報告書概要(欧文)」より
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[Publications] SAEKI,Y., OHNUKI,Y., YAMANE,A.and YANAGISAWA,K.: "Fiber types and their functional role in mammalian masticatory muscles"In Neurobiology of mastication-From molecular to systems approach (Eds.Nakamura, Y.and Sessle, B.J.), Elsevier, Amsterdam. 327-336 (1999)
Description
「研究成果報告書概要(欧文)」より
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[Publications] YAMANE,A., MAYO,M., SHULER,C., Crowe, D., OHUNUKI,Y., DALRYMPLE,K.and SAEKI,Y.: "Expression of myogenic regulatory factors during the development of mouse tongue striated muscle"Archs oral Biol.. 45(1). 71-78 (2000)
Description
「研究成果報告書概要(欧文)」より
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